表皮生长因子对新生大鼠高氧损伤肺组织EGFR及EGF mRNA表达的影响  

Effect of EGF on expression of EGFR and EGF mRNA in the injuried lung tissue of neonatal premature rats exposed to hyperoxia

作　　者：柳国胜[1] 刘海英[1] 邱锐琴[1] 聂川[2] 赵小朋[3] 康举龄[4] 关洁宾[5] 

机构地区：[1]暨南大学附属第一医院儿科,广东广州510632 [2]广东省妇幼保健院新生儿科,广东广州510010 [3]广州市妇婴医院新生儿科,广东广州510180 [4]暨南大学附属第一医院病理科,广东广州510632 [5]暨南大学医学院动物所,广东广州510630

出　　处：《中国病理生理杂志》2007年第7期1306-1310,共5页Chinese Journal of Pathophysiology

基　　金：广东省卫生厅科研基金资助项目(No2003379);国家教委教外司[1999]363号基金资助

摘　　要：目的:探讨外源性表皮生长因子(EGF)对新生大鼠高氧损伤肺组织EGFR及EGF mRNA表达的影响。方法:取胎龄21d剖宫产出生的新生Sprague dawley(SD)大鼠持续吸入95%的O2制作未成熟肺高氧损伤模型,随机分为高氧表皮生长因子(EGF)组和高氧生理盐水(NS)组,另设空气NS对照组;所有组按给药(或NS)时间分为3个亚组,即:a亚组(1-3d)、b亚组(4-6d)、c亚组(1-6d);各亚组均于生后3、7、14d分批处死取肺组织。应用免疫组化观察各组肺组织EGF-R的表达,RT-PCR方法检测EGF-mRNA的表达。结果:EGFmR-NA的表达随着日龄增加而递增,生后7、14d高氧组EGF-R及EGF mRNA的表达高于空气对照组,14dEGFa和c亚组EGF-R的表达均明显高于相应的高氧NS组(P<0.05),14d时EGF组内源性EGF mRNA的表达较NS组明显增加(P<0.01)。结论:早期应用EGF可促进肺泡上皮细胞EGF-R的表达,改善高氧所致肺发育受阻,对未成熟肺高氧损伤有一定的保护作用。AIM： To investigate the effect of exogenous epidermal growth factor（EGF） on expression of EGFR and EGF mRNA of the injuried lung tissue of neonatal premature rats exposed to hyperoxia. METHODS： The neonatal Sprague - Dawley rats of 21 - day gestational age model of hyperoxia induced lung injury were made by continually inhaling 95% oxygen. The model rats were divided into two groups randomly, the EGF trail group and the NS control group. The other rats were taken into the air control group. Each group was divided into three subsets ： a （ 1 - 3 days）, b （4 - 6 days） and c （1 -6 days） according to different application times of EGF or NS. Rats in sub groups were executed and the lung tissues were removed at postnatal 3th, 7th, 14th day respectively. Using immunohistochemistry method, the expression of EGF - R of lung tissues in different groups was observed, and the expression of EGF mRNA was determined by reverse transcriptase polymerase chain reaction （ RT - PCR）. RESULTS ： The expression of EGF mRNA increased by degrees following the increasing postnatal days. Compared with the air control group, the expression of EGF - R and EGF mRNA increased in the hyperxia group at 7th day and 14th day. The expression of EGF - R increased in corresponding hyperxia NS control group at 14th day （ P 〈 0. 05 ）. The expression of EGF - R in a and c subset of the EGF trail group all increased obviously compared with the corresponding hyperxia NS control group at 14th day （P 〈0.01 ）. CONCLUSION： EGF may facilitate the expression of EGF - R in alveolar epithelial cells, ameliorate the impaired development of lung resulted from hyperxia, and play a protective role in lung development from hyperoxia induced inhibition.

关 键 词：高氧症 肺损伤 表皮生长因子 大鼠 

分 类 号：R363[医药卫生—病理学]