反义c-myc寡核苷酸诱导骨肉瘤细胞MG-63凋亡的实验研究  

Induction of apoptosis by c-myc antisense oligonucleotide in osteosarcoma cell MG-63

作　　者：胡军[1] 王桂龙 向阳[2] 刘义[2] 陈一升[2] 丁茹虎[2] 

机构地区：[1]贵阳医学院附属医院病理科,贵州贵阳550004 [2]贵阳医学院附属医院骨科,贵州贵阳550004

出　　处：《中国病理生理杂志》2007年第7期1343-1346,共4页Chinese Journal of Pathophysiology

基　　金：贵州省教育厅自然科学研究资助项目(NoF2004-5)

摘　　要：目的:研究反义c-myc寡核苷酸诱导人骨肉瘤MG-63细胞凋亡的作用。方法:设计反义c-myc寡核苷酸片段,转染入人骨肉瘤MG-63细胞,通过MTT法、流式细胞仪、HE染色及透射电镜方法,观察和分析其对人骨肉瘤MG-63细胞作用的效果。结果:MTT法示反义c-myc寡核苷酸可抑制人骨肉瘤MG-63细胞的增殖,10.0μmol/L、作用48h效果最明显;流式细胞仪检测证实反义c-myc寡核苷酸(终浓度10.0μmol/L)诱导瘤细胞的凋亡率达37.92%,出现明显的凋亡峰,并可抑制c-myc基因蛋白的表达;细胞呈典型凋亡特征。结论:反义c-myc寡核苷酸能有效诱导人骨肉瘤MG-63细胞的凋亡。AIM ： To study the induction of apoptosis by c - myc antisense oligonucleotide in osteosarcoma cell （ MG - 63 ）. METHODS ： The designed c - myc antisense oligonucleotide fragment was transfected into human osteosarcoma MG - 63 cells. The cell growth and apoptosis were measured by the methods of MTT, FCM, HE staining and transmission electron microscopy. RESULTS ： The results showed that the proliferation of human osteosarcoma MG - 63 cells was inhibited and apoptotic rate was 37.92% when treated with c - myc antisense oligonucleotide at the does of 10. 0μmol/L for 48 h. c - myc antisense oligonucleotide （ 10. 0 μmol/L） also inhibited the expression of c - myc protein. CONCLUSION： c -myc antisense oligonucleotide is able to induce apoptosis in human osteosarcoma MG- 63 cells.

关 键 词：基因 c—myc 寡核苷酸类 反义 骨肉瘤 细胞凋亡 

分 类 号：R738.1[医药卫生—肿瘤]