8q24区域扩增靶基因在食管癌中的研究  

Investigation of the Key Gene in 8q24 Amplifications in Esophageal Cancer

作　　者：张旭[1] 黄晓平[1] 马国伟[1] 朱志华[1] 杨弘[1] 戎铁华[1] 

机构地区：[1]华南肿瘤学国家重点实验室中山大学肿瘤防治中心胸科,广州市510060

出　　处：《中国肿瘤临床》2007年第13期724-727,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金( 编号: 30572103);广东省自然科学基金( 编号: 5300808);中国博士后科学基金资助( 编号: 2005037612)

摘　　要：目的:染色体畸变(扩增和缺失)是癌发生和(或)发展的原因。在食管癌的发生发展中,8q24扩增是最常见的原因之一。本研究探讨8q24区域扩增的靶基因。方法:应用c-myc(8q24.12～q24.13)探针在食管癌组织中进行荧光原位杂交。另外,应用RT-PCR和(或)免疫组化的方法检测8q24区域几个基因c-myc、C8FW和NSE2在食管癌中的表达状态。结果:在食管癌中,8q24呈显著扩增。46例食管癌中只有4例在食管癌的癌巢中c-myc蛋白显示明显的表达;而且只有一小部分癌巢显示高表达。c-myc阳性染色主要见于食管癌细胞的细胞浆。在食管癌中,未发现C8FW的表达。NSE2在66%(39/59)的食管癌中显示高表达。结论:在食管癌中c-myc和C8FW可能不是8q24的扩增靶点。NSE2可能参与了食管癌的发生和(或)发展过程。NSE2是否为食管癌的扩增靶点需要进一步的研究来证实。Objective： Chromosomal aberrations including amplifications and deletions underlie the development of cancer. Amplification of 8q24 is one of the most frequent genetic events in esophageal cancer. Our study is designed to identify the key gene in 8q24 amplifications. Method： Fluorescent in situ hybridization using a c-myc（8q24.12-q24.13） probe was used to detect genetic amplifications in esophageal cancer specimens. The expression of several genes including c-myc, C8FW and NSE2 in the 8q24 region was detected via RT-PCR and immunohistochemical analysis. Result： Amplification of 8q24 was found in esophageal carcinomas. Only four of forty-six cases of esophageal malignancy showed significant upregulation in protein expression. Peculiarly, increased protein expression of c-myc was seen only in small portions of the lesions. Staining for c-myc was observed mainly in the cytoplasm of esophageal cancer cells. No expression of C8FW was detected in esophageal squamous cell carcinomas. Thirty-nine of fifty-nine （66%） cases showed increased expression of NSE2 in esophageal carcinomas. Conclusion： The results suggest that c-myc and C8FW are probably not the key gene in 8q24 amplifications in esophageal cancer. NSE2 might be involved in the development and/ or genesis of esophageal cancer. Whether NSE2 plays a pivotal role in esophageal cancer awaits further investigation.

关 键 词：食管癌 8q24 扩增 靶基因 

分 类 号：R735.1[医药卫生—肿瘤]