多种垂体外肿瘤细胞系ACTH基因表达的研究  

STUDIES OF ACTH GENE EXPRESSION IN EXTRA PITUITARY TUMOR CELL LINES

作　　者：王子梅[1,2] 陆召麟[1,2] 章静波[1,2] 刘鸿瑞[1,2] 郭爱丽[1,2] 党智勇[1,2] 汪迎[1,2] 

机构地区：[1]中国协和医科大学北京协和医院内分泌科 [2]中国医学科学院基础医学研究所

出　　处：《解剖学报》1997年第1期40-44,共5页Acta Anatomica Sinica

基　　金：CMB基金

摘　　要：用Ｎｏｒｔｈｅｒｎ杂交、免疫细胞化学和电镜技术，以垂体ＡＣＴＨ瘤细胞系（ＡｔＴ２０）为对照，从转录、翻译及亚细胞水平检测了５种垂体外不同病理组织来源的肿瘤细胞系ＡＣＴＨ基因表达，包括属于ＡＰＵＤ系统的两种人小细胞肺癌（ＳＣＬＣ－Ｈ６９和ＳＣＬＣ），３种非ＡＰＵＤ系统肿瘤：肺腺癌（ＡＧＺＹ８３－α、低分化胃粘液癌（ＭＧＣ－８０３）和肝癌（Ｂｅｌ－７４０２）。结果证实，（１）ＡＣＴＨ基因表达不仅存在于ＡＰＵＤ系统肿瘤，而且也存在于非ＡＰＵＤ系统肿瘤，如ＡＧＺＹ８３－α和ＭＧＣ－８０３。（２）不同细胞系中ＰＯＭＣ（ＡＣＴＨ前体）转录的ｍＲＮＡ表达长度不同（８００ｂｐ、１２００ｂｐ、１４００ｂｐ）。支持激素类物质合成在组织中普遍存在的假说。The pathogenesis of ectopic ACTH syndrome is still puzzling. In this study, Northern blot hybridization, immunocytochemistry and electron microscopy were used to examine ACTH gene expression in five extra pituitary tumor cell lines, including two human small cell lung cancer cell lines (SCLC H69 and SCLC) which belonged to APUD system and three non APUD human carcinoma cell lines (AGZY83 α, MGC 803 and Bel 7402), and the mouse pituitary ACTH secreting tumor cell line (AtT20) as a control. The results showed that, (1) ACTH was produced not only in APUD tissues but also in some non APUD tissues, such as lung adenocarcinoma(AGZY83 α), low differentiated mucoid adenocarcinoma of stomach (MGC 803). (2) Pro opiomelanocotin (POMC) mRNA expressing in different cell lines had different lengths(800bp, 1 200bp, 1 400bp). This results support the hypothesis of hormone producing in universal tissues, but there is different quality of ACTH gene expression

关 键 词：垂体外肿瘤 细胞系 ACTH 分泌 基因表达 

分 类 号：R736.402[医药卫生—肿瘤]