阿霉素诱导亲本 S-180 和抗药 S-180-R 细胞凋亡的差异  

DIFFERENCES OF ADRIAMYCIN INDUCED APOPTOSIS BETWEEN PARENTAL S 180 AND ADRIAMYCIN RESISTANT S 180 R CELL LINES

作　　者：李陆英[1,2,3] 韩复生[1,2,3] 

机构地区：[1]青岛医学院生物化学教研室 [2]中国中医研究院针灸研究所 [3]北京市肿瘤研究所

出　　处：《青岛医学院学报》1997年第1期48-49,共2页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省卫生厅科研基金

摘　　要：①目的探讨阿霉素对抗阿霉素细胞系Ｓ－１８０－Ｒ细胞凋亡的作用。②方法用低剂量（３．２５μｇ）和高剂量（６．５０μｇ）阿霉素分别处理亲本Ｓ－１８０和抗药Ｓ－１８０－Ｒ细胞腹腔接种的ＢＡＢＬ／Ｃ鼠，７２ｈ取腹水，用流式细胞仪测定细胞ＤＮＡ相对含量。③结果低剂量阿霉素７２ｈ使亲本Ｓ－１８０细胞出现６２．５％的凋亡，使抗药Ｓ－１８０－Ｒ仅出现１３．０％的凋亡细胞，高剂量阿霉素７２ｈ使亲本Ｓ－１８０细胞几乎全部死亡，使Ｓ－１８０－Ｒ出现２０．０％的细胞凋亡。低剂量阿霉素处理７２ｈ，亲本Ｓ－１８０细胞鼠腹腔出现大量淋巴细胞；抗药Ｓ－１８０－Ｒ细胞鼠腹腔只有少量淋巴细胞出现。高剂量阿霉素处理亲本Ｓ－１８０细胞仍保留很多淋巴细胞，其他Ｓ－１８０细胞几乎全部死亡，而抗药Ｓ－１８０－Ｒ细胞中淋巴细胞消失。④结论细胞抗凋亡是细胞抗药性的明显标志之一。抗阿霉素Ｓ－１８０－Ｒ细胞接种的小鼠腹腔内淋巴细胞的数量与阿霉素的剂量有关。Objective To study the effect of adriamycin on apoptosis of resistant cell line(S 180 R cells). Methods BABL/C mice were innoculated with parental S 180 and S 180 R cells in abdominal cavity. Then the mice were treated with both low dosage(3.25μg) and high dosage(6.50μg) of adriamycin. At the end of 72 h, the cells of parental S 180 and S 180 R were drawn from the abdominal cavity and analyzed by flow cytometer FACS 420, and the DNA content was determined. Results 62.5% of the parental S 180 cells and about 13.0% of the S 180 R cells were in apoptosis with low dosage of adriamycin at the end of 72h. The number of the abdominal cavity lymphocyte in parental S 180 cell innoculated mice was significantly increased, while the increase of lymphocyte in S 180 R cell in noculated mice was not so obvious. With high dosage of adriamycin, most of the S 180 cells were dead, only the lymphocytes were left in the abdominal cavity, about 20.0% of the S 180 R cells were in apoptosis, the lymphocytes in the abdominal cavity disappeared. Conclusions Resistance to cell apoptosis is one of the marks of cell resistance to drugs. The amount of lymphocytes in the abdominal cavity in S 180 R innoculated mice is related to the dosage of adriamycin.

关 键 词：抗癌药 阿霉素 抗药性 细胞凋亡 

分 类 号：R73－361[医药卫生—肿瘤] R979.14[医药卫生—临床医学]