ALA体内转化PP Ⅸ光动力学疗法对肿瘤细胞形态学的影响  

Influence of photodynamic therapy of endogenous ALA-induced PP Ⅸ on tumor morphological pathology

作　　者：龚涌灵[1] 李宏杰[2] 路红社[3] 董荣春[4] 

机构地区：[1]第二军医大学长海医院消化内科,上海200433 [2]复旦大学生物化学系 [3]第二军医大学长海医院神经外科 [4]第二军医大学基础医学部病理解剖学教研室

出　　处：《第二军医大学学报》1997年第1期53-55,共3页Academic Journal of Second Military Medical University

摘　　要：目的:评价δ－氨基－γ－酮戊酸（ALA）体内转化原叶啉Ⅸ（PPⅨ）光动力学疗法（PDT）杀伤肿瘤细胞的实际意义。方法：荷H22肝癌昆明小鼠，应用ALA在动物体内转化成PPⅨ，观察光敏治疗后肿瘤组织的形态学改变。结果：PDT治疗后肿瘤细胞明显变性、解离、胞核固缩，瘤组织弥漫性出血、坏死。超微结构主要显示线粒体的损伤和破坏。结论：ALA对H22肝癌的PDT主要是对肿瘤组织癌细胞线粒体的破坏。To evaluate the practical photodynamic therapy (PTD) efficiency on tumor of en-dogenous ALA-induced PP Ⅸ. Methods: Morphoraglcal alterations were observed after PDT 0f protopor-phyrin Ⅸ (PP Ⅸ) by application of endogenous 6-aminolaevulinic acid(ALA) in Kunming mice bearing H22hepatic carcinoma. Results: After PDT, the tumor cells showed degeneration, deformation and nuclearnecrosis and the tumor tissues were defused with hemorrhage and necrosis. The ultrastructural pathologyindicated that mitochondria in tumor cells were injured and destroyed. C0nclusi0n: The pathological alter-ations suggest that PDT of ALA on H22 hepatic carcinoma was conducted by the destruction of mitochon-dria.

关 键 词：肿瘤 ALA 光动力学疗法 原卟啉 

分 类 号：R730.5[医药卫生—肿瘤]