部分喉切除术后喉狭窄瘢痕组织中TGF-β_1和α-SMA的表达  

Expression of TGF-β1 and α-SMA in Laryngeal Stenosis Scar after Partial Laryngectomy

作　　者：吴旋[1] 苏振忠[1] 蒋爱云[1] 赖英荣[2] 雷文斌[1] 

机构地区：[1]中山大学附属第一医院耳鼻喉科医院,广东广州510080 [2]中山大学附属第一医院病理科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2007年第4期439-442,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学研究基金(31733);广东省科技计划项目(2006B60501022)

摘　　要：【目的】研究喉狭窄瘢痕组织的病理变化,了解转化生长因子β1(TGF-β1)和α-平滑肌肌动蛋白(α-SMA)在喉狭窄瘢痕组织中表达及意义。【方法】垂直喉部分切除术后2～3个月出现喉狭窄10例和未狭窄16例喉瘢痕肉芽组织为实验组及10例正常声带组织为对照组,进行免疫组化染色,观察组织切片中TGF-β1和α-SMA的表达,并在KONTRON IBAS2.5全自动图像分析系统下进行阳性细胞计数。【结果】TGF-β1阳性表达主要分布于炎症细胞及成纤维细胞的胞浆,α-SMA阳性表达的主要分布于肌成纤维细胞胞浆。比较非狭窄喉瘢痕组织及正常声带组织,喉狭窄瘢痕组织的TGF-β1和α-SMA的表达均明显增强,阳性细胞数量差异有统计学意义(P<0.05)。【结论】喉狭窄瘢痕组织的TGFβ1及α-SMA高表达,这些改变可能是喉狭窄发生重要内在因素。[Objective] To explore the significance of the expression of transforming growth factor β1 （TG-β1） and α-smooth muscle actin （α-SMA） in the laryngeal stricture scar by the study of the pathological features of laryngeal stenosis scar. [Methods] The laryngeal tissues were 10 human surgical specimens of laryngeal stenosis scar occurred after 2-3 months of vertical partial laryngectomy and 16 human surgical specimens of laryngeal scar without-stenosis after vertical partial laryngectomy; 10 human normal vocal folds were conducted as control. Expression of TGF-β1 and α-SMA were observed by immunohistochemistry staining respectively in laryngeal tissue sections. The amount of positive cells were analyzed with KONTRON IBAS 2.5 automatic image analysis system. [Results]TGF-β1 positive staining appeared in much inflammation and fibroblast cells, and α-SMA positive staining appeared in muscle fibroblast cells in inhesion layer, with a dense distributing in laryngeal stenosis scar. The count of TGF-β1 and α-SMA positive staining cell in laryngeal stenosis group were significantly higher than those in the normal and no stenosis group （P 〈 0.05）. [Conclusions] There were obviously high expression TGF-β1 and α-SMA in the laryngeal stenosis tissue, which may play an important role in the formation of laryngeal stenosis SCar.

关 键 词：垂直喉部分切除 喉狭窄 瘢痕/病理学 转化生长因子Β 平滑肌动蛋白 

分 类 号：R767[医药卫生—耳鼻咽喉科]