肺癌E-cadherin基因启动子CpG岛甲基化的研究  

The Study of CpG Island Methylation of E-cadherin Gene Promoter in Lung Cancer

作　　者：王红兵[1] 苗慧[1] 张敬川[1] 祖茂衡[1] 蔡晓敏 吴秀英 胡西旦 迪力努 陈春玲 

机构地区：[1]徐州医学院附属医院,221002 [2]新疆伊犁州新华医院,835000

出　　处：《实用癌症杂志》2007年第4期357-359,共3页The Practical Journal of Cancer

基　　金：新疆伊犁哈萨克自治州科技攻关和重点科技项目(YZ200601025);徐州医学院附属医院课题(2006022)

摘　　要：目的检测肺癌组织、相应的癌旁组织及正常肺组织中E-cadherin基因启动子CpG岛甲基化水平,探讨肺癌的发病机制。方法采用甲基化特异性PCR技术检测22例肺癌组织、相应的癌旁组织和9例正常肺组织中E-cad-herin基因启动子CpG岛甲基化水平。结果肺癌中E-cadherin基因启动子CpG岛完全甲基化率为13.6%(3/22),部分甲基化率为27.3%(6/22),总甲基化率为40.9%(9/22),显著高于相应癌旁组织中该基因的甲基化率9.1%(2/22)。9例正常肺组织中该基因未发生甲基化。此外,E-cadherin基因启动子CpG岛异常甲基化与患者性别、年龄无关,甲基化的发生率随着肿瘤的分期早晚有上升的趋势。结论E-cadherin基因启动子CpG岛的异常甲基化是肺癌发生中的常见分子事件,可能参与了肺癌的发生发展过程。Objective To detect the CpG island methylation status of E-cadherin gene promoter in lung cancer , paired adjacent cancerous tissues and normal lung tissues, and to explore the mechanism of lung carcinogenesis. Methods The tissue specimens from 22 cases of lung cancer,adjacent cancerous tissues and 9 cases of normal lung tissues were examined CpG island methylation of E-cadherin gene promoter by methylation specific PCR. Results The positive rate of E-cadherin hypermethylation in lung tumor tissues was significantly higher than that in the adjacent cancerous tissues（40.9% vs 9.1%, P 〈0.05 ）. The incidence of promoter hypermethylation was not examined in 9 normal lung tissues. Anymore, hypermethylation of E-cadheHn was not associated with gender and age. The incidence ratio of promoter hypermethylation of E-cadherin is ascendant with the carcinomas becoming much later. Conclusion Methylation of E-cadherin gene is a common molecule event in lung cancer and play a role in the development and progression of lung cancer.

关 键 词：肺癌 上皮细胞钙粘蛋白 CPG岛 甲基化 甲基化特异性聚合酶链反应 

分 类 号：R734.2[医药卫生—肿瘤]