阻断氯离子通道对小鼠成牙本质细胞MDPC-23生物学特性影响的研究  

Effects after blocked chloride channels on mouse odontoblast-like cells (MDPC-23) in Vitro

作　　者：侯晋[1] 段小红[1] 司徒镇强[1] 

机构地区：[1]第四军医大学口腔医学院口腔生物教研室,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2007年第6期316-319,共4页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(30371540)

摘　　要：目的:研究氯离子通道对小鼠成牙本质细胞MDPC-23生物学特性的影响。方法:应用MTT、生化分析、荧光染色等方法,观察NPPB阻断氯离子通道之后MDPC-23细胞增殖,ALP活性以及形成细胞外矿化基质的能力等方面的变化。结果:氯离子通道被阻断后,细胞的增殖速度减慢,培养上清中的碱性磷酸酶活性明显增高,而且细胞外矿化结节的形成时间比对照组缩短。结论:阻断氯离子通道可抑制成牙本质细胞MDPC-23的增殖,但对其矿化有一定的促进作用。AIM ： To investigate the effects of chloride channels on the biological characteristic of mouse odontoblast-like cells, MDPC -23, in vitro. METHODS： NPPB, a kind of chloride channel blocker, was used to block the chloride channels. The cells proliferation was detected by MTT assay. Biochemical analysis and immunofluo- rescence methods were used to study the effects on the alkaline phosphatase activity （ALP） and mineralization. RESULTS： The cells in treatment group proliferated slower than those in the control group. The ALP activity of the treatment group was higher than that of the control group . There was significant difference of ALP activity between the two groups （ P 〈0.01 ）. The cells of treatment group formed mineralized nodus after continuouse cultured for 21 days. However, the cells of control group took 28 days to form the nodus. CONCLUSIONS ： These results suggested that the chloride channels may be involved in the proliferation and differentiation of odontoblast - like cells, MDPC - 23.

关 键 词：氯离子通道 成牙本质细胞 细胞增殖 碱性磷酸酶 

分 类 号：R780.2[医药卫生—口腔医学]