破骨细胞形成过程中在牵张力下的差异蛋白分析  

Analysis of differential expression of proteins in the formation of osteoclast by mechanical strain

作　　者：王艳[1] 段银钟[1] 李永明[1] 路凡[2] 阮禹松[3] 

机构地区：[1]第四军医大学口腔医学院 [2]第四军医大学生化教研室,陕西西安710032 [3]西安交通大学生命科学与技术学院,陕西西安710049

出　　处：《牙体牙髓牙周病学杂志》2007年第6期328-332,共5页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(30570455)

摘　　要：目的:应用比较蛋白质组学的方法分析破骨细胞在牵张应力作用下蛋白质的差异表达,为阐明牵张力对破骨细胞的形成机制提供分子基础。方法:利用人骨髓单核细胞,牵张应力加载培养体系诱导培养破骨细胞,分为加力组与对照组。应用双向电泳分离以上两组样本可溶性蛋白质。应用基质辅助激光解吸电离飞行时间质谱获得差异蛋白点的肽质量指纹图谱;通过SWISS-PROT数据库鉴定蛋白质。结果:得到两张2-DE图谱,初步筛选出在牵张力的作用下,有13个蛋白点的表达存在明显差异。其中新增蛋白点1个,缺失7个,2个蛋白点表达发生明显上调,3个蛋白点发生明显下调。经质谱分析,初步鉴定了2种蛋白质。结论:应用2-DE及MALDI-TOF-MS方法分离并初步鉴定了2种蛋白质,为研究牵张力在破骨细胞形成过程中的机制以及正畸骨改建的发生机理提供线索。AIM： To investigate the effect of the cyclic tension force on the osteoclast formation in human bone marrow culture, so as to learn about the mechanisms of bone remodeling under mechanical strain. METHODS： Osteoclasts were cultured in marrow cultures plated on flexible membranes. Comparative proteomic approach based on two dimensional gel electrophoresis, Mass- spectrum identification and bioinformatics analysis was used to study the difference of strain and control group. RESULTS：According to 2 - DE results, 13 differential protein spots were found by image analysis. Among which 1 spot was new, 7 spots were disappeared, 2 spots were increased, 3 spots were decreased. Whereafter 2 spots were identified initially by mapping peptide mass fingerprinting of protein spots by MALDI -TOF- MS. CONCLUSION： 2 spots were identified from protein database. These results are helpful for further study on the mechanisms of bone remodeling and osteoclast formation under mechanical strain.

关 键 词：牵张力 破骨细胞 差异蛋白组学 双向凝胶电泳 基质辅助激光解吸电离/飞行时间质谱 

分 类 号：R780.2[医药卫生—口腔医学]