转cry1Ab基因玉米对粘虫的抗性评价  

Resistance evaluation of transgenic Bt maize to Oriental armyworm

作　　者：常雪[1] 常雪艳[1] 何康来[1] 王振营[1] 白树雄[1] 

机构地区：[1]中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京100094

出　　处：《植物保护学报》2007年第3期225-228,共4页Journal of Plant Protection

基　　金："863"课题(2002AA212161)资助

摘　　要：粘虫Mythimna separata（Walker）是我国玉米上的重要害虫。转Bt基因玉米为防治粘虫提供了新的途径。从室内生测和田间抗性鉴定分别研究了转cry1Ab基因抗虫玉米YieldGard对粘虫的杀虫效果。用4～7叶期转cry1Ab基因玉米心叶室内饲养1～3龄粘虫幼虫3、7和10天的死亡率分别为39．0％～92．0％、96．0％～98．0％和97．0％～100％，第14天全部死亡，而对照的死亡率分别为3．0％～8．0％、6．0％～20．0％和11．0％～28．0％，差异极显著。取食转cry1Ab基因玉米的幼虫不能正常生长发育，存活10天的幼虫其体重仅为取食对照玉米的9．0％。田间玉米4～5叶期，分别接1～6龄粘虫幼虫于转cry1Ab基因和非转基因玉米心叶。转cry1Ab基因玉米上各龄期幼虫平均存活率仅为0．0～6．7％，极显著低于非转基因玉米的8．3％～80．0％。转cry1Ab基因玉米的危害级别0．1～0．7，显著小于非转基因玉米的2．1～7．3。结果说明转cry1Ab基因玉米YieldGard对粘虫各龄幼虫有很高的杀虫作用和田间控制效果，同时能有效保护玉米免受危害。The Oriental armyworm, Mythimna separata Planting transgenic Bt maize is a new way for the control （Walker）, is an important insect pest of maize. of corn borers. Monsanto＇s YieldGard transgenic Bt maize expressing Cry1Ab protein and a non-Bt control were evaluated through laboratory bioassays by exposing 1st-3rd instar larvae of Oriental army worm directly to whorl leaves. The 1st -3rd instar larvae fed on Bt maize leaves could lead to 39.0%-92.0%, 96.0%-98.0%, and 97.0%- 100% mortalities within 3, 7, and 10 days, respectively. All larvae could not survive longer than 14 days. There were significant difference compared with only 3.0% - 8.0% , 6.0% - 20.0% , and 11.0% - 28.0% mortalities for the non-Bt control. The development of larvae was also severely inhibited when feeding on Bt maize. Larvae survived 10 days on Bt maize leaves weighted only 9% of that on non-Bt control. Field trials were also conducted with artificial infestations of 1 st -6th instar larvae into the maize whorl at V4 -V7 stage, respectively. There were only 0.0 -6.7% of survival on Bt maize. In contrast, there were 8.3% -80.0% of survival on non-Bt control, which were significantly higher than Bt maize. Damage ratings of Bt maize were 0. 1 - 0.7, which were significantly lower than 2. 1 - 7.3 of non-Bt control. These results indicated that Yield- Gard could provide good controls to Oriental armyworm and protect maize well from the damage.

关 键 词：BT玉米 粘虫 抗性 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]