牛磺酸对牛体外受精早期胚胎发育的影响  

Effect of Taurine on the Development of Bovine Early Embryo Following of IVF

作　　者：袁水桥[1] 罗光彬[2] 李东全[2] 王冰松[1] 文江[1] 

机构地区：[1]沈阳二O四医院生殖医学研究中心,辽宁沈阳110043 [2]沈阳农业大学畜牧兽医学院动物胚胎工程实验室,辽宁沈阳110161

出　　处：《西北农业学报》2007年第4期59-62,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：沈阳市科学技术局攻关项目(1032051-1-02)

摘　　要：研究了牛体外受精后早期胚胎体外发育时向其基础培养液中加入牛磺酸对胚胎桑椹胚率、囊胚率和孵化囊胚率的影响。试验一:以TCM-199+10%FBS为基础培养液(对照组),再加入7、14 mmol/L的牛磺酸(试验组),试验组与对照组的桑椹胚率分别为48.1%、47.4%和43.2%;囊胚率分别为26.4%、22.3%和21.0%;孵化囊胚率分别为21.8%、18.7%和0%。试验二:IVF后2细胞、4～8细胞及8～16细胞期,在基础培养液中分别添加7 mmol/L的牛磺酸时,桑椹胚率分别为48.7%、57.1%、52.0%,囊胚率分别为25.1%、30.7%、27.9%,孵化囊胚率分别为25.9%、28.6%、25.0%;而添加14 mmol/L牛磺酸组时,桑椹胚率分别为50.4%、56.4%、55.4%,囊胚率分别为26.5%、31.3%、27.7%,孵化囊胚率分别为27.5%、31.1%、29.9%。结果表明,体外发育培养液中添加7 mmol/L牛磺酸可显著提高桑椹胚率和囊胚率(P<0.05),并且在4～8细胞期添加14 mmol/L牛磺酸最为合适。The effect of in-vitro-fertilized embryos cultured in basic culture medium with different tau- rine concentration on the morulae rate, blastocyst rate, and hatched blastocyst were detected and com- pared. Experimnet 1： The morulae rates of the treatment groups with 7 and 14 mmol/L taurine were 48.1%, 47.4%, respectively, had no difference with the control without taurine in TCM-199＋10% FBS（43.2%） （P〈0. 05）. The blastocyst rate were 26.4%、22.3% and 21.0% each, and the group with 7 mmol/L taurine was significantly higher than the other groups（P〈0.05）. There was no emergence hatched blastocyst in the control groups, the hatched blastocyst rates of treatment groups were 21.8% 、18. 7%. Experiment2： when adding to 7 mmol/L taurine in the basic medium at the 2-cell period,4-8 cell period and 8-16 cell period, Morulae rates were 48. 7%,57.1% and 52.0% each. The blastocyst rate were 25. 1%, 30. 7% and 27. 9% each. The hatched blastocyst rates were 25. 9%, 28. 6% and 25.0% each. when adding 14 mmol/L taurine in the basic medium at the 2-cell period,4-8 cell period and 8-16 cell period, however, Morulae rates were 50.4% ,56.4% and 55.4% each. The blastocyst rate were 26.5%, 31.3% and 27.7%each. The hatched blastocyst rates were 27.5%, 31.1% and 29.9% each.. These showed prove the morulae rate and blastocyst at 4-8 cell period got the best effect. supplementation of 7 mmol/L taurine in rate significantly （P〈O. 05）, and adding the medium could imto14 mmol/L taurine

关 键 词：牛 牛磺酸 体外受精 

分 类 号：S823[农业科学—畜牧学]