抗人CD_3、CD_7—PAPs免疫毒素对T细胞的联合杀伤效应  

Combined killing efficacy of anti-CD_3, CD_7 McAb-PAPs to T lymphocyte

作　　者：李官成[1] 林学颜[2] 周其锋[2] 

机构地区：[1]遵义医学院免疫学教研室 [2]中山医科大学免疫学教研室

出　　处：《遵义医学院学报》1997年第1期6-10,共5页Journal of Zunyi Medical University

基　　金：中山医科大学国内访问学者课题;由美国中华医学基金

摘　　要：免疫毒素（IT）杀伤试验分8组进行。主要采用改进的MTT法测定不同浓度的抗CD3-PAPs、抗CD7-PAPs、抗CD3-PAPS十抗CD7—PAPs、多抗AHTG—PAPs杀伤T细胞的效率，同时以B细胞作非靶细胞对照。结果证实，两种抗人T细胞单抗IT和多抗IT均具有特异性杀伤T细胞的作用。比较杀伤率发现，单抗IT特异性高，杀伤力也较强;多抗IT杀伤力强，但特异性较差;而两种单抗IT合用则杀伤力进一步提高，并能保持其特异性。结果提示，我们制备的两种单抗IT可用于体夕P清除T细胞作异体骨髓和／或脐带血移植;两者联合应用可能其协同作用效果更好。There were eight groups in the cytotoxicity test, i. e. anti-CD3-PAPs,anti--CD7-PAPs, anti-CD3-PAPs + anti-CD7-PAPs, AHTG-PAPs, NIgG--PAPs,antiCD3 McAb,anti-CD3 McAb+PAPs and free PAPs. Each was separately added into T and B lymphocytes,cultured in 96-well plates. An improved MTT assay was performed,as references to determine the efficacy and specificity of immunotoxins. The results showed that the groups of anti--CD3-PAPs,anti--CD7--PAPs and AHTG-PAPs had a selective cytotoxicity to T lymphocytes. McAb immunotoxins showed a higher specificity but lower cytotoxicity than PcAb immunotoxin. The mixture of antiCD3-PAPs with anti-CD7-PAPs showed a similar cytotoxic efficacy and higher specificity compared with AHTG-PAPs. The results indicated that the combination o f McAb immunotoxins would be more effective than any one alone in the targeting therapy,especially in bone marrow or umbilical cord blood transplantation as purging reagents.

关 键 词：T细胞 免疫毒素 联合杀伤效应 

分 类 号：R392.32[医药卫生—免疫学]