培养基Grace's对棉铃虫细胞HzAm1培养的改进  

Improvement of Medium Grace's for the Cultivation of Helicoverpa Armigera Cells HzAm1

作　　者：张佑红[1] 陈燕[1] 杜进[2] 朱雄伟[1] 秦琴[1] 赵亮[1] 吴元欣[1] 

机构地区：[1]武汉工程大学湖北省新型反应器与绿色化学工艺重点实验室,湖北武汉430073 [2]北京大学医学部,北京100083

出　　处：《化学与生物工程》2007年第7期49-51,共3页Chemistry & Bioengineering

基　　金：武汉市科技攻关项目(20052002044);湖北省重点实验室开放基金资助项目(RCT2004004)

摘　　要：昆虫细胞-杆状病毒表达体系给生物杀虫剂的工业化生产带来了希望。为了提高杆状病毒在昆虫细胞中的复制量并大规模生产生物杀虫剂,高性能、低成本培养基的选取十分必要。将Grace's培养基与IPL-41及Schneider's培养基的组分进行比较后,以Grace's为基础,向其中加入酵母提取物、水解乳蛋白、氨基酸以及钼、锰等微量元素进行改善,用于培养细胞HzAm1,最大细胞浓度提高了190.2%,改进效果明显。Insect cell-baculovirus expression system is emerging a hopeful future for the industrial production of bio-insecticides. In order to improve the baculovirus replication in insect cells and be feasible in bio-insecticide production on large scale, the selection of high-performance and low-cost medium is quite necessary. In this study, compared to media IPL-41 and Schneider＇s, medium Grace＇s is improved through the addition of yeast extract, lactalbumin hydrolysate, amino acids, manganese, molybdenum and etc. It is indicated that the insect cells HzAml concentration is greatly raised by 190.2% when cultivated with this modified Grace＇s medium.

关 键 词：棉铃虫细胞 培养基 氨基酸 微量元素 

分 类 号：Q813[生物学—生物工程]