荧光标记拔牙创口骨愈合过程中矿化沉积速度的研究  

The effect of estrogen on bone wound healing of extracted sockets in osteoporotic rats: fluorescence labeling observation

作　　者：李晓红[1] 朱晓姝[1] 赵献银[1] 苏兴宇[1] 

机构地区：[1]西安交通大学口腔医院种植中心,西安710004

出　　处：《中国骨质疏松杂志》2007年第7期486-489,共4页Chinese Journal of Osteoporosis

摘　　要：目的研究骨质疏松及雌激素替代治疗对拔牙创骨愈合的影响。方法选用12周龄雌性SD大鼠,随机分为假手术对照组和实验组,实验组行双侧卵巢切除术,对照组行假去势手术。术后8周将实验组随机分为骨质疏松组(OP)和雌激素治疗组,同时拔除所有大鼠左侧上颌第1磨牙。磨牙拔除术后7、30、60天分3批处死大鼠。上颌拔牙创口处制作不脱钙切片,行组织学及荧光观察。结果拔牙后7天时,3组荧光表现基本相似;拔牙后30天及60天骨质疏松组拔牙创骨矿沉积率明显低于假手术对照组及雌激素治疗组,雌激素治疗组骨矿沉积率低于假手术对照组。结论OP对拔牙后早期的新骨形成骨的矿化影响不大,但在拔牙创愈合中晚期可明显减少拔牙创新骨矿化。雌激素应用可明显减缓OP对拔牙创内新骨骨矿化的影响。Objective To investigate the effect of estrogen on bone wound healing progress of extracted sockets in postmenopausal osteoporosis cases. Methods 12-week-old female SD rats were divided into two groups. Ovariectomy was performed on experimental group while control group received sham-operation. Eight weeks after surgery we divided the experimental group into osteoporosis group and estrogen therapy group. At the same time all the left maxillary morlar teeth were extracted. The rats were killed on the 7th, 30th and 60th day respectively after extracted surgery. Undecalcified sections of first molar extraction area were examined histologically to clarify the changes of new bone around the first molar extraction area by fluorescence observation. Result The changes by the fluorescence observation among three groups had no obvious difference on the 7th day. While on the 30th day and on the 60th day, the MAR was better in control group than in estrogen group and the OP group was the lowest. Conclusion OP has little influence on the mineral appositional rate during the earlier period of bone wound healing around the extracted sockets. OP car, obviously decrease MAR during the middle and late period of bone wound healing.

关 键 词：骨质疏松症 拔牙创 雌激素 骨标记组织学 

分 类 号：R782.11[医药卫生—口腔医学]