谷氨酰胺复方制剂对晕船大鼠脑体疲劳的影响  

Effects of compound preparation of glutamine on mental and physical fatigue induced by experimental seasickness in rats

作　　者：祝路民[1] 李正银[1] 郭俊生[1] 秦海宏[1] 

机构地区：[1]第二军医大学海医系军队卫生学教研室,上海200433

出　　处：《中华航海医学与高气压医学杂志》2007年第3期137-140,共4页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：海军医学专项基金(HJJ2005-05)

摘　　要：目的探讨谷氨酰胺复方制剂对晕船后大鼠脑体疲劳的影响。方法取112只SD雄性大鼠,随机分为正常对照组(N)、生理盐水组(S)、谷氨酰胺组(G)、谷氨酰胺+维生素组(GV)、谷氨酰胺+维生素+支链氨基酸组(GVB),谷氨酰胺+维生素+酪氨酸组(GVT),谷氨酰胺+维生素+支链氨基酸+酪氨酸组(GVBT),每组又随机分为A组和B组,每组8只。连续灌胃21 d,模拟晕船刺激3 d,取每组中的A组,测定其游泳时间;B组测定其学习记忆能力和生化指标。结果与S组相比,N组(P<0.05)、GVB组和GVBT组(P<0.01)的游泳时间显著延长;N组和GVT组的学习记忆能力显著增强(P<0.05);N组、GV组、GVB组和GVBT组的血清尿素氮的含量显著降低(P<0.05);GV组,GVB组,GVBT组(P<0.05)和GVT组(P<0.01)的脑乳酸的含量显著下降;N组、G组、GV组和GVB组的血乳酸含量显著降低(P<0.05);G组、GV组、GVB组和GVT组的肌糖原的含量显著升高(P<0.05和P<0.01);N组(P<0.05),G组、GV组、GVB组、GVT组和GVBT组(P<0.01)的肝糖原含量显著升高。结论谷氨酰胺复方制剂具有提高晕船大鼠脑体功能的作用,其作用机制可能与增加肝糖原储备,改善脑的有氧代谢,以及提高骨骼细胞对能量物质的利用有关。Objective To explore effects of compound preparation of glutamine on mental and physical fatigue in rats with experimental seasickness. Methods One hundred and twelve SD male rats were divided into seven groups randomly ： normal control （ N ）, normal saline control （ S ） , glutamine supplemented group （ G ） , glutamine and vitamins supplemented group （GV）, glutamine, vitamins and branched chain amino acid supplemented group（ GVB ） , glutamine, vitamins and tyrosine group （ GVT ）, glntamine, vitamins, branched chain amino acid and tyrosine group（GVBT）. Each of group was randomly divided into group A and B, consisting of 8 rats. After they had received experimental treatment for 21 days, the swimming time of group A was examined after simulated seasickness stimulation for 3 days continually. The group B was used to analyze the content of glycogen,lactate and serum urea nitrogen（ SUN）, as well as the ability of learning and memory. Results The compound preparation of glutamine could increase the learning and memory abilities, and the swimming time of rats remarkably： swimming time of the N,GVB and GVBT group was prolonged compared with the S group（P 〈0.01 ）, meanwhile the learning and memory abilities of the N and GVT group were boosted compared with the S group （ P 〈 0. 05 ） . Moreover, the contents of glycogen in the liver and muscle of supplement groups were increased by from 2 to 3 times, while the concentrations of lactate in the serum and brain were decreased significantly （ P 〈 0.05 ） , as well as the concentration of serum urea nitrogen did （ P 〈 0. 05 ）. Conclusions The glutamine compound preparation might improve experimental seasickness rats physical and mental performances. Its possible mechanism might be related to the increase of liver glycogen storage, the amelioration of aerobic metabolism in brain, and the improvement in muscular ability to utilize the energy.

关 键 词：晕船 疲劳 谷氨酰胺复合制剂 学习记忆能力 糖原 乳酸 血清尿素氮 

分 类 号：R96[医药卫生—药理学] R83[医药卫生—药学]