左旋四氢帕马汀对大鼠肾缺血再灌注损伤的保护作用  

Protective Effect of L-tetrahydropalmatine on Renal Ischemia Reperfusion Injury in Rats

作　　者：刘家兰[1] 李德清[1] 

机构地区：[1]湖北民族学院医学院实验中心,湖北恩施445000

出　　处：《湖北民族学院学报（医学版）》2007年第2期25-27,共3页Journal of Hubei Minzu University(Medical Edition)

摘　　要：目的探讨左旋四氢帕马汀(L-THP)对大鼠肾缺血再灌注损伤的影响。方法建立肾缺血再灌注损伤大鼠模型,34只大鼠分为治疗组(n=10)和模型组(n=16)。另设假手术组(n=8)作为对照。治疗组于肾动脉夹闭时静脉给予L-THP60mg/kg,再灌注时又给予原药量的1/2;模型组同时给予等量0.9%氯化钠溶液。观察肾组织中P-选择素、细胞间粘附分子(ICAM-1)的表达及丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性变化,检测血清尿素氮(BUN)和肌酐(Cr)的含量。结果缺血再灌注后,模型组大鼠肾组织中P-选择素、ICAM-1明显表达,MDA含量增高,SOD活性下降,BUN和Cr含量明显上升;治疗组肾组织中P-选择素、ICAM-1表达受到抑制;相对模型组MDA含量降低,SOD活性增高,BUN和Cr含量降低。结论P-选择素和ICAM-1参与肾缺血再灌注损伤机制,L-THP明显减轻脂质过氧化反应,对肾缺血再灌注损伤大鼠具有保护作用。Objective To investigate the effect and protective effect of L - tetrahydropalmatine（ L -THP）on renal ischemia- reperfusion injury in rat. Methods Rat models of renal ischemia- reperfusion were established,and 34 male rats were randomly divided into 3 groups,treatment group with L - THP （n = 10）, renal ischemia - reperfusion injury model group （n = 16） and sham operation group （n = 8） as the control. L- THP （60 mg/kg） was given intravenously during clamping renal artery in treatment group and one half of the dose was infused after opening the clamp during reperfusion. In modelgroup equal amount of 0.9% sodium chloride solution was given. In the sham operation group （control group）, the rats were anesthetized with laparotomy but without blocking renal blood flow. The P -selectin and ICAM -1 expression, malo- nyldialdehyde （MDA） contents and super oxide dismutase（SOD） activities in renal tissues, level of serum blood urea nitrogen（BUN） and creatinine（Cr） were detected. Results After renal ischemia reperfusion injury, there were significantly up- regulated expressions of Pselectin and ICAM -1 ,with increased M DA content and declined SOD activity inrenal tissue,and significant increase in BUN and Cr content in model group, and in treatment group with L - THP these effects were significantly depressed as compared with model group. Conclusion It is thought that the adhesion molecules P - selectin and ICAM - 1 take part in the process of renal ischemia- reperfusion injury, and L-THP significantly reduce the reaction of lipid peroxidation with a protective effect on renal ischemia - reperfusion injury

关 键 词：左旋四氢帕马汀 粘附分子 肾缺血再灌注损伤 

分 类 号：R364.2[医药卫生—病理学]