大蒜素在体外对棘阿米巴的杀伤作用  

The Effect of Allitridium against Acanthamoeba in vitro

作　　者：郑善子[1] 王月华[2] 

机构地区：[1]延边大学基础医学院,吉林延吉133000 [2]吉林医药学院,吉林吉林132013

出　　处：《时珍国医国药》2007年第7期1661-1662,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的观察大蒜素在体外对棘阿米巴的杀伤作用。方法将棘阿米巴用不同浓度的大蒜素30℃作用3,24 h后,在倒置显微镜下观察棘阿米巴的形态和其细胞增殖速率的变化。结果大蒜素浓度分别以0.01,0.1,1,10 g/L处理3 h时,棘阿米巴由滋养体转化为包囊,1,10 g/L处理3,24 h后观察到大量细胞分裂碎片;细胞生长曲线与对照组比较,0.1 g/L组增殖速率正常,而浓度高于1 g/L以上时培养4 d仍未见细胞增殖。结论在体外大蒜素对棘阿米巴生长有明显抑制和杀伤作用。Objective To detect the effect of allitridium on Acanthamoeba spp. Methods Acanthamoeba spp. was cultured at 30℃ with different concentration of allitridium, the influence of allitridium on the morphological feature, proliferation speed was detected. Results At concentrations of 0.01, 0. 1, 1.0, 10 g/L Allitridium cultured for 3h, the Acanthamoeba trophozoltes changed to cysts and in the 10 g/L allitridium group cell destroying could be detected. Cell proliferation could not detected. Conclusion Allitridium is effective to destroy Acanthamoeba in vitro.

关 键 词：棘阿米巴 大蒜素 体外 

分 类 号：R285.5[医药卫生—中药学]