大蒜素对小鼠膀胱癌的体内及体外抗肿瘤效果的实验研究  

The Experimental Research on the Antitumor Effect of Allicin on Murine Bladder

作　　者：伍少雄[1] 黄冬[1] 周飞[1] 

机构地区：[1]广东医学院附属医院,广东湛江524001

出　　处：《时珍国医国药》2007年第7期1667-1668,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的了解大蒜素对小鼠膀胱肿瘤的抗肿瘤效果与机制。方法通过MTT实验评定了大蒜素的直接细胞毒活性,动物实验显示大蒜素在体内有明显的抗肿瘤效果,并用LDH释放法测细胞毒活性。结果大蒜素对膀胱肿瘤有直接细胞毒作用。大剂量治疗组与对照组相比,肿瘤的生长速度明显受到抑制(P<0.01),大蒜素治疗后产生了针对B16肿瘤细胞的淋巴细胞。结论大蒜素对膀胱肿瘤有明显的抗肿瘤作用。这种效果可能与直接细胞毒作用和免疫反应有关。大蒜素可能是一种有效的表浅膀胱癌腔内治疗药物。Objective To understand anti-tumor effect and mechanism of allicin on murine bladder. Methods The direct inhibition effects of allicin in vitro was evaluated by MTT Assay. Anti-tumor effect of allicin in vivro was determined by animal experiment, CTL activity of lymphocyte was measured by the release of endogenous lactate dehydrogenase（LDH）. Results Allicin had significant direct cytotoxicity to bladder cancer cells. In high dose group,it showed a marked delay in the appearance and growth of tumors after s.c. injection compared with control group（P 〈0.01 ）, The body generated the cytotoxic lymphocyte responses to B16 tumor cells after treated with allicin, Conclusion Allicin has a marked anti-tumor effect on bladder tumor. This effect may be related to direct cytotoxicity and immune response, It may be an effective intravesical treatment agent for superficial bladder cancer.

关 键 词：大蒜素 抗肿瘤 膀胱肿瘤 

分 类 号：R285.5[医药卫生—中药学]