青藏高原高原鼠兔Na^+,K^+-ATP酶β2亚基基因编码区的克隆与分析  

Cloning and Analysis of the Coding cDNA Sequence of Na^+,K^+-ATPase β2 Subunit of Ochotona princeps(Pika)Lived in Qinghai-Tibet Plateau

作　　者：尚爱加[1] 陈婷方[1] 周定标[2] 白振忠[3] 高艳[1] 侯冰[1] 格日力[3] 张成岗[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]解放军总医院神经外科,北京100853 [3]青海大学医学院高原医学中心,青海西宁810001

出　　处：《生物技术通讯》2007年第4期553-556,共4页Letters in Biotechnology

基　　金：国家自然科学基金重大研究计划(90208017);国家自然科学基金面上项目(30571903;30400465;30300358)

摘　　要：目的:克隆青藏高原高原鼠兔Na+,K+-ATP酶β2亚基(ATP1B2)的基因编码区,并分析其序列特征,以揭示高原鼠兔低氧适应的分子基础。方法:采用RT-PCR技术从高原鼠兔脑组织中扩增出ATP1B2基因编码区cDNA序列并进行序列测定,采用生物信息学技术对其进行分析。结果:ATP1B2基因编码区由873bp组成,编码290个氨基酸残基。序列分析结果显示,高原鼠兔ATP1B2编码区的核酸序列与兔、人、牛、大鼠、小鼠及狗分别有99%、93%、91%、91%、90%和90%的同源性。结论:克隆出青藏高原高原鼠兔ATP1B2基因编码区,为进一步了解高原鼠兔低氧适应的分子机制提供了基础。Objective： To clone and analysis the coding region of Na^＋,K^＋-ATPase β2 Subunit（ATP1B2） of Ochotona princeps（pika）. Methods： The coding region of ATP1B2 of pika lived in Qinghai-Tibet plateau was identified using RT-PCR technique and analyzed with bioinformatic approaches. Results： The coding region of pika ATP1B2 comprises 873 bp and codes for 290 amino acids. Sequence analysis showed that the nucleic acid sequence of pika ATP1B2 shares 99%, 93%, 91%, 91%, 90% and 90% identity with that of rabbit, human, cow, rat, mouse and dog, respectively. Conclusion： Coding region of cDNA sequence of pika ATP1B2 was successfully identified, which is important for us to understand the molecular mechanism for these animals with high adaptation ability to the hvlaoxia environment.

关 键 词：高原鼠兔Na^+ K^+-ATP酶 基因克隆 低氧适应 

分 类 号：Q78[生物学—分子生物学]