人14-3-3蛋白家族的生物信息学分析  

Bioinformatic Analysis of 14-3-3 Protein Family of Homo sapiens

作　　者：袁保梅[1] 昝玉玺[2] 王天云[2] 张俊河[2] 杨保胜[2] 

机构地区：[1]郑州大学生物工程系,河南郑州450052 [2]新乡医学院生物化学与分子生物学教研室,河南新乡453003

出　　处：《生物技术通讯》2007年第4期587-589,共3页Letters in Biotechnology

基　　金：河南省自然科学基金项目(0511042300)

摘　　要：目的:分析人14-3-3蛋白家族的同源性及分子进化关系。方法:利用已公布的人基因组数据库,采用BLASTN程序检索人14-3-3蛋白家族各成员的编码基因和假基因,并利用DNAMAN软件进行序列联配,绘制其分子进化树。结果:该家族半数成员具有多个假基因序列,为返转座类型假基因。进化分析表明该家族有共同的祖先,可归为3个亚类。结论:人14-3-3蛋白家族每个成员长期进化所形成的多样性提示其功能具有独特性。Objective： To determine the genomic structure of 14-3-3 protein family of Homo sapiens, and to analyze its molecular evolution. Methods： Based on the published genome database of 14-3-3 protein family of H.sapiens, the coding genes and pseudogenes of all 14-3-3 members were retrieved by using BLASTN software, and their genomic structures were analyzed. All the sequences were multialigned with DNAMAN software and the molecular evolution tree was drawn. Results： Most of all 14-3-3 members have pseudogenes which were the retrotransposons of coding genes. The evolution of this gene family showed that they had the common ancestor and clustered as three subgroup. Conclusion： As a result of long time evolution, the diversity of this family suggested that each member might have its specific functions.

关 键 词：14-3-3蛋白家族 基因家族 进化 生物信息学 

分 类 号：Q811.4[生物学—生物工程] R394[医药卫生—医学遗传学]