氧化胁迫在硝基苯致小鼠神经毒性中的作用  

The Effect of Oxidative Intimidation on Nitrobenzene Induced Neurotoxicity in Mouse

作　　者：邢厚娟[1] 王海波[1] 王敏[1] 徐世文[1] 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030

出　　处：《生物技术通讯》2007年第4期616-618,共3页Letters in Biotechnology

基　　金：黑龙江省教育厅资助项目(YJSCX2007-0043HLJ)

摘　　要：目的:探讨氧化胁迫在硝基苯致小鼠神经毒性中的作用。方法:用不同剂量的硝基苯对小鼠进行灌胃染毒,每天1次,共30d;用碱性羟胺比色法测定小鼠脑乙酰胆碱酯酶活性,用试剂盒检测脑抗氧化功能。结果:试验组小鼠的中毒症状随着染毒剂量的增加和时间的延长而越来越明显;乙酰胆碱酯酶活性降低;与对照组相比,脑各项抗氧化指标均表现出显著或极显著差异。结论:氧化胁迫在硝基苯发挥神经毒性过程中起重要作用。Objective： To study the effect of oxidative intimidation on nitrobenzene induced neurotoxicity in mouse. Methods： The mouse were administered with various doses of nitrobenzene for 30 days. The activity of acetylcholinesterase was measured by the alkalescence hydroxylamine colorimetry, and the antioxidative capacities of brain were measured by kit. Results： With the increasing of doses and time the toxic symptom of test group appeared earlier and more seriously. Conccntration of brain＇s antioxidate in the test group were all remarkably highcr than that of the normal control group, with the activity of acetylcholinesterase decreased. Conclusion： Oxidation intimidate play an important role in the procedure of nitrobenzene induced neurotoxicity.

关 键 词：硝基苯 神经毒性 乙酰胆碱酯酶 抗氧化功能 

分 类 号：X503.22[环境科学与工程—环境工程] Q42[生物学—神经生物学]