LipofectAMINE2000与Fugene6转染细胞的效率比较  

The Transfection Efficiency Comparison of LipofectAMINE2000 and Fugene6

作　　者：张宝[1,2] 霍霞[1] 徐锡金[1] 齐宗利[1] 郑谨[1] 彭琳[1] 

机构地区：[1]汕头大学医学院中心实验室,广东省免疫病理学重点实验室 [2]南方医科大学生物化学教研室,广东广州510515

出　　处：《生物技术通讯》2007年第4期638-640,共3页Letters in Biotechnology

基　　金：中国博士后科学基金项目(2005038187)

摘　　要：目的:比较LipofectAMINE2000与Fugene6转染细胞的效果。方法:将含有Firefly和Renilla荧光素酶基因的质粒分别用LipofectAMINE2000和Fugene6转染293T、HepG2和DLD-1细胞,于48h后裂解细胞测定荧光素酶活性。结果:在293T细胞中,LipofectAMINE2000转染组的萤火虫(Firefly)和Renilla荧光素酶活性分别是Fugene6转染组的6.5和5.6倍(P<0.005);在HepG2细胞中,LipofectAMINE2000转染组的Firefly和Renilla荧光素酶活性分别是Fugene6转染组的44和49倍(P<0.001);而在DLD-1细胞中,两者无差别。结论:转染试剂LipofectAMINE2000和Fugene6对不同细胞的转染效果存在差异,当进行转染实验时,对于不同的细胞须根据情况进行选择。Objective： To compare the transfection efficiency of LipofectAMINE2000 and Fugene6. Methods： The plasmids containing the Firefly luciferase and Renilla luciferase gene were transfected into 293T, HepG2, DLD-1 cells using LipofectAMINE2000 and Fugene6 respectively. After 48 h transfection, ceils were lysed and the luciferase activity was detected. Results： In 293T cells, the Firefly and Renilla luciferase activity of LipofectAMINE2000 transfection group were as more 6.5, 5.6 fold as that of Fugene6 transfection group （P〈O.005）; while in HepG2 cells, the activity of LipofectAMINE2000 transfection group were as more 44, 49 times as Fugene6 transfection group （P〈O.001）; but in DLD-1 cells, there was no difference between two groups. Conclusion： There is significant difference between LipofectAMINE2000 and Fugene6 for transfecting different cells. That might be needed to choose proper transfection agents depending on the type of cells.

关 键 词：LipofectAMINE2000 Fugene6 转染 

分 类 号：Q78[生物学—分子生物学]