抗C-erbB-2单克隆抗体在荷人乳腺癌裸鼠体内的预定位显像  

Radioimmunoimaging study using pretargeting system on ^(153)Sm labeled anti-C-erbB-2 monoclonal antibody in nude mice grafted with human breast carcinoma

作　　者：常伟勤[1] 许天敏[1] 王文加[2] 王丁丁[2] 苏曼曼[2] 焦平[2] 张赢予[2] 

机构地区：[1]吉林大学第二医院,吉林长春130041 [2]吉林大学再生医学研究所

出　　处：《中国实验诊断学》2007年第7期857-859,共3页Chinese Journal of Laboratory Diagnosis

基　　金：吉林大学青年教师基金项目资助

摘　　要：目的以153Sm标记的抗C-erbB-2单克隆抗体对荷人乳腺癌移植瘤裸鼠进行肿瘤预定位显像的研究。为乳腺癌早期诊断提供一种新方法,并为其临床应用提供实验依据。方法实验I组每只裸鼠采用三步预定位法从尾静脉注射7.4 MBq/0.1 ml的153Sm-DTPA-C-erbB-2 McAb实验II组与II组分别直接从尾静脉注射7.4 MBq/0.1 ml的135 Sm-C-erbB-2及135Sm-IgG并于于注射后2、4、8、24h行全身SPECT显像。于4、82、4 h分批处死裸鼠并测定分别在肿瘤、血液、心脏、肺等重要脏器单位重量的放射性比值(%ID/g)。结果在运用预定位技术加药的实验I组在给药后2小时开始有抗体在肿瘤组织浓聚,在8小时时达到最高,与实验II组直接给予抗体比较,血液本底低,两组比较差异有显著性(P<0.05)。结论应用预定位技术的实验组,瘤/血液比值高,血液本底低,周围正常组织吸收剂量低,显像效果好。Objective To study using pretargeting system on radioimmunoirmaging of ^153Sm labeled anti- C-erbB-2 monodonal antibody and normal mice IgG in nude mice with haman breast carcinoma and provide the basis for clinical use . Methods Group I 3-step pretargeting injected into the tail vein with 7.4 MBg/0.1 ml labeled antibody. Group Ⅱ and Ⅲ injected directly into the tail vein with 7.4 MBg/0. 1ml labeled antibody. SPECT were measured after 2,4,8,24 h interval of injecion . Percent injected dosage of every gram tissue（ % ID/g）were measured after 4,8,24 h interval to death .Results The tumor blood ratio of groupI reached 6.53, 8.11,9.25 at 2,4,8 hours respectively.Radioactivity concertratoin peaked at 8 h in tumor site. the tamor/blood ratio of groupI was higher than groupⅡ and groupⅢ. （ P 〈 0.05）. Conclusion Pretargeting system on radioimmunoimaging improves the amounts of locatingon toamors and yields a soecific enhancing effect and also improve image quality.

关 键 词：预定位技术放射免疫显像 乳腺癌 抗C-erbB-2单克隆抗体 

分 类 号：R737.9[医药卫生—肿瘤]