宫颈癌前病变中HPV16/18与p16的关系  

An associantion between human papillomavirus 16/18 and p16 protein expression in neoplastic cervical lesions

作　　者：陈映缄[1] 黄杰雄[1] 邱前程[1] 曾庆纯[1] 谢斌[1] 

机构地区：[1]汕头大学医学院第一附属医院妇产科,广东汕头515041

出　　处：《中国实验诊断学》2007年第7期900-902,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的研究宫颈癌的各个阶段中人乳头状瘤病毒(Human papillomavirus,HPV)和抑癌基因p16的表达,探讨HPV致癌的机理。方法利用免疫组织化学技术检测20例正常宫颈组织、30例宫颈低度鳞状上皮内病变(LSIL)、30例宫颈高度鳞状上皮内病变(HSIL)和20例宫颈鳞状细胞癌中PHV16/18和p16的表达水平。结果正常宫颈组织没有HPV16/18和p16的表达;LSIL、HSIL和宫颈鳞状细胞癌中HPV16/18的阳性率分别为16.7%(5/20)、53.3%(16/30)和75%(15/20);p16的阳性率分别为36.7%(11/30)、73.3%(22/30)和100%(20/20)。HPV16/18和p16在HSIL的表达均明显高于LSIL。16例HPV16/18阳性的HSIL中14例发现p16的高表达。结论宫颈癌前病变存在p16的过度表达;HPV16/18阳性的宫颈癌及HSIL常伴随p16蛋白的高表达,提示HPV16/18有可能引起自然抑癌基因p16突变,而导致细胞癌变。Objective Tested the expression of human papillomavirus 16/18 and p16 in neoplastic cervical lesions to investigate the carcinogenic mechanism of HPV.Methods Immunohistochemical analysis of p16 and HPV16/18 were performed on 20 normal cervical tissues,30 low-grade squamons intraepithehal lesions（LSIL）, 30 high-grade squamons intraepithelial lesions（HSIL） and 20 carcinomas.Results The expression of HPV16/18 in LSIL,HSIL and carcinomas were 6.7%（5/20）,53.3%（16/30）和 75%（15/ 20） ,respectively;and p16 were 36.7%（11/30）,73.3%（22/30）和 100%（20/20） respectively. Never HPV16/18 nor p16 overexpression in normal cervic tissues. Both HPV16/18 and p16 expression in HSIL were higher than in LSIL. As for HSIL cases, 14 cases showed p16 overexpression in 16 HPV16/18 positive cases. Conclusion The results suggested that HPV16/18 may be cause mutation of p16 gene.

关 键 词：HPV P16 宫颈 鳞状上皮内病变(SIL) 

分 类 号：R737.33[医药卫生—肿瘤]