隔离肺灌注模型的动物实验研究  

Exprimental Study of Isolated Lung Perfusion Animal Model

作　　者：辛华[1] 闫继东[1] 高楠[1] 刘威[2] 黄海波[3] 

机构地区：[1]吉林大学中日联谊医院,吉林长春130033 [2]吉林省中医中药研究院 [3]青岛大学医学院附属烟台毓璜顶医院

出　　处：《中国实验诊断学》2007年第7期952-953,共2页Chinese Journal of Laboratory Diagnosis

基　　金：本研究为吉林省科技发展计划项目资助(20030543)

摘　　要：目的成功建立隔离肺灌注化疗的动物模型。方法建立兔隔离单肺灌注系统,使用阿霉素进行灌注化疗,并应用荧光分光光度法检测双肺及心、肝、肾组织中阿霉素含量及肺湿/干重量比。结果隔离灌注肺的阿霉素含量较对照肺及心、肝、肾组织显著升高;而肺湿/干重量比则无明显差别。结论我们使用的隔离肺灌注系统可在肺组织局部得到较高的化疗药物浓度,且未出现急性肺损伤,使用该模型治疗肺癌及肺转移癌应可取得较好效果。Objective To establish the animal model of isolated lung perfusion.Methods Successfully establish a rabbit model of single lung perfusion system. The lung is isolated and the perfusion is limited between the pulmonary artery and pulmonary vein. Use this model and perfuse with Adriamycin（ADM） .Then examine the ADM level in the tissue of two lungs,heart,liver and kidney by the fluorescence spectrophotometry. Exmine the wet/dry lung weight. Results The ADM level in the isolated lung is extremly higher than that in the contrast lung, heart, liver, kidney. The wet to dry weight of two lungs have no difference. Conclusion Higher drug level can get in the isolated lung without acute lung injury. This animal model is safe and effective.

关 键 词：隔离肺 灌注 阿霉素 肺癌 

分 类 号：R-332[医药卫生]