低氧诱导因子-1α干扰RNA对血管内皮生长因子mRNA表达的抑制作用  

The inhibition effect of HIF-1α siRNA on VEGF mRNA expression in vitro

作　　者：许惠卓[1] 刘双珍[1] 夏晓波[1] 熊思齐[1] 王育科[1] 李岩 

机构地区：[1]中南大学湘雅医院眼科,湖南长沙410008 [2]Texas大学M.D.Anderson癌症研究中心,美国houseontx77030

出　　处：《眼视光学杂志》2007年第4期228-231,共4页Chinese Journal of Optometry & Ophthalmology

基　　金：国家自然科学基金资助项目(030471853)

摘　　要：目的探讨低氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)特异性小片段干扰RNA表达载体pSU-PERH1-siHIF1α对低氧诱导因子-1α和血管内皮生长因子(vas-cular endothelial growth factor,VEGF)mRNA表达的抑制作用。方法构建HIF-1α特异性小片段干扰RNA表达载体pSUPERH1-siHIF1α,通过脂质体Lipofectamine2000分别介导pSUPERH1-siHIF1α转染低氧状态下培养的人低分化鼻咽鳞癌细胞(nasopharyngeal carcinoma cell line,CNE2),对照组转染pSUPER空载体,采用半定量RT-PCR方法检测HIF-1αmRNA和VEGFmRNA的表达。结果转染pSUPERH1-siHIF1α的CNE2细胞中HIF-1αmRNA和VEGFmRNA的表达较对照组分别降低79.4%和65.7%。结论HIF-1α特异性小片段干扰RNA表达载体pSUPERH1-siHIF1α能明显抑制HIF-1αmRNA和VEGFmRNA的表达。Objective To evaluate the inhibition effect of the HIF-1α specific siRNA expression vector pSUPER^H1-SiHIF1α on HIF-1α and VEGF mRNA expression in vitro. Methods The HIF-1α specific siRNA expression vector pSUPER^H1-SiHIF1α was constructed by gene recombination and then transfeeted into a human CNE2 cell line euhured under hypoxia conditions （1%） with Lipofectamine 2000. The transfection of the pSUPER vector was used as a control. HIF-1α and VEGF mRNA expression were detected by semi-quantitative RT-PCR. Results The expression of HIF-1α and VEGF mRNA of CNE2 transfected with pSUPER^H1-SiHIF1α was dramatically lower than that of the control transfection. Conclusion HIF-1α specific siRNA expression plasmid pSUPER^H1-SiHIF1α can specifically down-regulate the expression of HIF-1α and VEGF mRNA in CNE2 under hypoxia conditions.

关 键 词：RNA干扰 低氧诱导因子-1 血管内皮生长因子 低分化鼻咽鳞癌细胞 

分 类 号：R774.1[医药卫生—眼科]