无机阳离子对胞内粗酶液降解体系中芘萃取过程的破乳研究  

The demulsification study on the extraction process of pyrene from biodegradation system of endoenzyme fluid of bacteria

作　　者：王蕾[1] 聂麦茜[1] 王志盈[1] 吴蔓莉[1] 苏君梅[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西西安710055

出　　处：《西安建筑科技大学学报（自然科学版）》2007年第3期353-357,共5页Journal of Xi'an University of Architecture & Technology(Natural Science Edition)

基　　金：陕西省教育厅科研计划项目(自然科学专项)(04JK157)

摘　　要：对CB9#菌体粗酶液降解体系中芘萃取的防乳化及破乳过程进行了研究,定性的实验结果表明Hg^2＋和Ba^2＋具有明显防乳化或破乳作用.定量的破乳脱水实验结果表明加入Hg^2＋破乳效果最好,其浓度在300-350 mg/L范围内,萃取时几乎不出现乳化层,脱水率达99%;Ba^2＋效果不明显,但其浓度为1000 mg/L时,静置后期,脱水速度加快,乳化层逐渐消失,萃取静置25 min后脱水率也达到99%.从酶促降解体系中萃取芘时,加入Hg^2＋芘回收率93.04%,Ba2＋回收率则为91.02%,空白对照仅为84.85%.在无机离子存在下萃取获得的芘有机溶液中,芘吸收峰无变化,说明外加离子对物质芘的紫外光谱无影响.The qualitative test results showed that Hg^2＋ and Ba^2＋ had the obvious demulsifying effects on the extracting process of the biodegradation system of coarse endoenzyme fluid of bacteria CB9# . The results of dehydration showed that Hg^2＋ was the most effective for the demulsification process of the systems, when the concentration of Hg^2＋remains within 300-350 mg/L, and the emulsification layer rarely appeared within the separating funnel, and the dehydrating rate could reach 99. 0% during the extraction. The demulsifying effect of Ba^2＋ was not evident, but when its concentration was about 1000 mg/L, the dehydration of the emulsification layer quickly followed, and the dehydrating rate could also reach 99. 0% within 25min as the extracting system is kept in still state. The extracting recovery rate of pyrene was 93.04% with Hg^2＋ as the demulsifying reagent, and 91.02% for Ba^2＋ existing system, 84.85% for the blank comparing one.

关 键 词：酶促降解体系 芘 萃取 破乳 

分 类 号：X132[环境科学与工程—环境科学]