去甲斑蝥素对HO-8910PM细胞中NAG-1表达的影响  

Effect of norcantharidin on the expression of NAG-1 in HO-8910PM cell line

作　　者：何太平[1] 莫丽儿[2] 梁念慈[1] 

机构地区：[1]广东医学院生物化学与分子生物学研究所,湛江524023 [2]广东天然药物研究与开发重点实验室,湛江524023

出　　处：《中国药科大学学报》2007年第4期356-359,共4页Journal of China Pharmaceutical University

基　　金：广东省医学科研基金资助项目(No.B2005104);广东省中医药局课题资助项目(No.1060044)~~

摘　　要：目的:研究去甲斑蝥素对人高转移卵巢癌HO-8910PM细胞中非类固醇抗炎药物激活基因(non-steroidal anti-in-flammatory drug-activated gene,NAG-1)表达的影响,探讨其抗肿瘤的作用机理。方法:应用肿瘤基因芯片、荧光定量实时PCR和Western blot法检测去甲斑蝥素对NAG-1表达的影响。结果:去甲斑蝥素能明显上调NAG-1表达,3种方法检测去甲斑蝥素上调HO-8910PM细胞中NAG-1表达的倍数分别为6.69、8.33±0.76和16.00倍。结论:去甲斑蝥素抗肿瘤作用与NAG-1表达有关。Aim：To investigate effect and its mechanism of norcantharidin on the expression of nonsteroidal anti-infla mmatory drug-activated gene（NAG-1） in the highly metastatic ovarian carcinoma HO-8910PM cells. Methods： The expression level of NAG-1 was assessed by using the mieroarray chip. Quantitative real-time fluorescent PCR assay and Western blot were used to verify the effect of norcantharidin on NAG-1 expression. Results： Microarray chip, fluores- cent quantitative real-time PCR and Western blot results showed that norantharidin significantly up-regulated the expression of NAG- 1, the relative mRNA and protein level of NAG- 1 were 6.69, 8.33± 0.76, 14.00 fold of control group, respectively. Conclusion： Anti-tumor activity and its mechanism of norcantharidin in HO-8910PM cells are associated with the expression of NAG-1.

关 键 词：去甲斑蝥素 卵巢癌 非类固醇抗炎药物激活基因 表达 

分 类 号：R737.31[医药卫生—肿瘤]