木糖氧化无色杆菌反硝化亚种细菌的分离鉴定及其菲降解特性研究  

Isolation of Achromobacter xylosoxidans subsp. denitrificans and it's degradation of phenanthrene

作　　者：武凤霞[1] 范丙全[2] 刘建玲[1] 

机构地区：[1]西北大学生命学院,陕西西安710069 [2]中国农业科学院农业资源与农业区划研究所,北京100081

出　　处：《植物营养与肥料学报》2007年第4期725-729,共5页Journal of Plant Nutrition and Fertilizers

基　　金：国家重点基础研究发展规划(973)项目(2002CB410809)资助

摘　　要：利用选择性富集培养及升华法,从石油污染的土壤中分离到2株菲降解细菌,它们在以菲为唯一碳源的培养基上生长良好。应用BIOLOG细菌鉴定系统和分子生物学方法对两株细菌进行鉴定,两株菌分别为坚强芽孢杆菌(Bacillus firmus)和木糖氧化无色杆菌反硝化亚种(Achromobacter xylosoxidanssub sp.denitrificans),两株菌均具有邻苯二酚氧化酶活性。两株细菌在液体培养条件下都表现较强降解菲的能力,液体培养60 h约90%的加入菲被降解。通过测定液体培养基中菲浓度和菌体密度变化发现,菌株降解菲的量与其生长密度相关;随着菌体浓度(吸光度)的增加,代谢底物菲的浓度明显降低,两株菌混合使用能够大幅度提高降解菲的能力。Isolation of polycyclic aromatic hydrocarbon-degrading bacteria was conducted in plate medium using selective enrichment culture. Two isolates of FC31 and FC32 were obtained by the means of sublimation from petroleum-contaminated soil. Both strains grew well on sole carbon of phenanthrene and degraded it efficiently. FC31 was identified as Bacillus firmus and FC32 as Achromobacter xylosoxidans subsp. and Biolog-GN profile. Both strains were of catechol dioxygenase denitrificans based on analysis of 16S rDNA sequence activity and higher phenanthrene-degrading capacities in the broth assay with about 90% of phenanthrene added being degraded during 60 hours incubation. The quantity of phenanthrene degradation was correlated with the OD value. The results indicated that the ability of phenanthrene-degrading was strongly increased when they were used as a mixture.

关 键 词：多环芳烃 菲 降解 坚强芽孢杆菌 木糖氧化无色杆菌反硝化亚种 

分 类 号：X172[环境科学与工程—环境科学]