TK/GCV、CD/5-Fc系统对结肠癌肝转移抑制作用的实验研究  

Investigation of inhibition of liver metastasis of colon carcinoma on the nude mice models with the system of TK/GCV and CD/5-Fc

作　　者：牛洪欣[1] 何庆泗[1] 魏冬[2] 谭伟[3] 高静[1] 

机构地区：[1]山东大学齐鲁医院普外科,山东济南250012 [2]卫生部北京医院普外科,北京100730 [3]济南市中心医院普外科,山东济南250013

出　　处：《中国现代普通外科进展》2007年第3期203-207,共5页Chinese Journal of Current Advances in General Surgery

基　　金：山东省自然科学基金资助项目(Y2003C05)

摘　　要：目的:研究胸苷激酶/丙氧鸟苷(TK/GCV)系统和胞嘧啶脱氨酶/5-氟胞嘧啶(CD/5-Fc)系统对结肠癌肝转移的抑制作用。方法:将40只裸鼠随机分为四组:对照组、TK组、CD组、TK-CD组,每组10只。脾内注射法建立结肠癌肝转移动物模型。对照组:脾内注射SW480细胞,腹腔注射生理盐水;TK组:脾内注射SW480/TK-CD细胞,腹腔注射GCV;CD组:脾内注射SW480/TK-CD细胞,腹腔注射5-Fc;TK-CD组:脾内注射SW480/TK-CD细胞,腹腔注射GCV、5-Fc。观察各组裸小鼠的肝转移率、肝转移瘤数目、电镜下肿瘤组织病理学变化、肿瘤细胞凋亡指数(AI)、生存期等指标。结果:各治疗组裸鼠肝转移的发生率下降,平均肝转移瘤数减少,其动物的生存期延长,肝转移瘤内的癌细胞凋亡率增高。联合基因治疗组效果更好,且TK/GCV与CD/5-Fc有交互协同作用。结论:TK/GCV、CD/5-Fc系统联合应用具有协同效应,可有效地抑制结肠癌肝转移的形成。Objectives： To explore the inhibition effect of TK/GCV and CD/5-FC system on liv-er metastasis of colon carcinoma. Methods： Forty nude mice were divided into 4 groups randomly, which were the control group, TK group, CD group and TK-CD group with 10 nude mice in each group. The mice in the control group were injected SW480 cells in the spleen and NS into the peri-toneal cavity. The mice in other groups were all injected SW480/TK-CD cells in the spleen. And GVC,5-Fc, GVC＋5F-c were injected into the peritoneal cavity of the mice in the TK group, CD group and TK-CD group respectively. Liver metastasis rate, the number of liver metastasis, the pathologi- cal changes of the tumor tissues under the electronic microscope, tumor cell＇s apoptotic index and life span and other indexes of nude mice in four groups were analyzed. Results： Liver metastasis rate of nude mice in every treatment group was lower than that in control group. Compared with control group, average number of liver metastasis decreased, life span of nude mice was prolonged, and the apoptosis rate of cancer cell in liver metastasis increased. The combined genes in the TK-CD group worked more markedly, and there was mutual effect between TK/GCV and CD/5-Fc. Conclusions： Synergistic effect was acquired combining TK/GCV with CD/5-FC system and it in-hibited the formation of liver metastasis of colon carcinoma efficiently.

关 键 词：结肠肿瘤 直肠肿瘤 肝转移 裸鼠 胸苷激酶 丙氧鸟苷 胞嘧啶脱氨酶 5-氟胞嘧啶基因治疗 

分 类 号：R735.7[医药卫生—肿瘤]