介入治疗对兔VX2肝癌MMP-2/PCNA/VEGF表达的影响及其意义  

Siginificance and effects of interventional treatments on MMP-2/PCNA/VEGF gene expression in the rabbit VX2 liver tumor

作　　者：李春海[1] 冯艳[1] 刘春喜[2] 谷涛[1] 李彩霞[1] 

机构地区：[1]山东大学齐鲁医院影像中心,山东济南250012 [2]教育部和卫生部心血管重构与功能研究重点实验室,山东济南250012

出　　处：《中国现代普通外科进展》2007年第3期208-212,共5页Chinese Journal of Current Advances in General Surgery

基　　金：山东省卫生厅资助项目(2001CAICJA12)。

摘　　要：目的:模拟不同方法介入治疗对兔VX2肝癌基质金属蛋白酶(MMP-2)、增殖细胞核抗原(PC-NA)、血管内皮生长因子(VEGF)表达的影响。方法:将VX2瘤细胞接种于40只新西兰大白兔肝左叶,建立VX2肝癌模型,随机分为4组,每组10只。介入治疗前MRI测量并记录肿瘤直径。经股动脉途径行肝固有动脉插管,分别注入生理盐水(对照组)、水化碘油(A组)、Ad-p53(B组)、Ad-p53+水化碘油(C组)。术后1周处死动物,取肿瘤组织制作石蜡切片(HE)染色,镜下观察肿瘤组织坏死情况。免疫组化方法测定MMP-2/PCNA/VEGF的表达。结果:经肝动脉插管介入治疗1周后,A、B、C组肿瘤生长受到明显抑制,与对照组比较,P均<0.05。单纯碘油栓塞后,肿瘤区的MMP-2、PCNA及VEGF的表达略有升高,与对照组相比P>0.05;B组与C组的MMP-2、PCNA及VEGF的表达阳性率降低,与对照组相比,P均<0.05;有转移的MMP-2、PCNA及VEGF的表达阳性率均高于无转移者(P<0.05);MMP-2与VEGF、PCNA之间之间有相关性(P<0.05)。结论:碘油+Ad-p53可抑制肿瘤的生长,抑制肿瘤新生血管形成,减少转移。MMP-2、PCNA、VEGF的增高预示着肿瘤的高转移、高增殖能力,肿瘤血管的高形成能力。Objectives：To investigate the effect of interventional treatments on the expression of matrix metalloprotein-2 （MMP-2） / proliferating cell nuclear antigen （PCNA） / vascular endothelial growth factor （VEGF）. Methods：VX2 carcinoma cells were surgically implanted into the left liver lobe of 40 New Zealand white rabbits, which were divided into 4 groups randomly. Physiological saline （compared group）, Lipiodol（group 1）, Ad-p53（group 2） and Lipiodol ＋ Ad-p53 （group 3） were infused in different groups via common hepatic artery. The rabbits were killed and the livers were re-sected one week later. MMP-2, PCNA and VEGF of the tumor were examined by immunohisto-chemistry. REsults： Growth of the tumor was suppressed in therapeutic groups. The positive rates of MMP-2, PCNA and VEGF were slightly higher in single lipiodol embolization group, but with no significant vs saline group （P〉0.05）. The positive rates of MMP-2, PCNA and VEGF in groups 2 and 3 were significantly lower than those of compared group （P〈0.05）. The positive rates of MMP-2, PC- NA and VEGF of the rabbits with metastasis were markedly higher than those of no-metastasis rab- bits （P〈0.05）. The correlations among MMP-2 and VEGF, MMP-2 and PCNA were found in this study （P〈0.05）. Conclusion： The growth, metastasis and vascular formation of rabbit liver tumors could be partially inhibited by Ad-p53 and lipiodol embolization. The high positive rates of MMP-2, PCNA and VEGF indicated high metastasis, proliferation potentiality and vascular formation poten-tiality.

关 键 词：介入疗法 肝肿瘤 VX2细胞株 兔 基质金属蛋白酶 增殖细胞核抗原 血管内皮生长因子 

分 类 号：R735.7[医药卫生—肿瘤]