犬瘟热病毒F基因克隆及序列分析  

Cloning and Sequenceing of F Gene of Canine distemper virus

作　　者：高华峰[1] 朱建波[1] 赵文华[1] 姚俊[1] 张念祖[1] 

机构地区：[1]农业部热带亚热带动物病毒病重点实验室,云南昆明650224

出　　处：《动物医学进展》2007年第7期40-43,共4页Progress In Veterinary Medicine

摘　　要：从犬瘟热病毒检测阳性的3份（KM1、KM2、KM3）病料中进行F基因的克隆测序,并与其他5株代表性参考毒株的同一基因序列进行比较（国内外流行毒株以及疫苗毒株）。结果表明,KM1与KM2有95.7%的核苷酸同源性,其氨基酸序列完全相同;KM3株与KM1有1个氨基酸的差异,与参考的5株犬瘟热病毒的F基因核苷酸和氨基酸分析同源性分别为91.1%~96.5%和96.8%~100%。Suspicious canine distemper virus （CDV） samples collected from Kunming were amplified by u- sing RT-PCR, in which 3 positive samples were detected. 3 strains of canine distemper virus （named KM1, KM2, KM3） were used for protein F gene cloning and sequeneeing and then compared with 5 representative reference strains. The results showed that the F gene from strain of KM1 and KM2 have the same amino acid sequence, but the strain of KM3 have a amino acid difference compared with the strain of KM1. At the nueleotide level KM1 shared the homologue of 91.1% to 96.5% and at the amino acid level, the homologue is from 96.8% to 100% compared with related viruses.

关 键 词：犬瘟热病毒 F基因 融合基因 序列分析 

分 类 号：S852.659.5[农业科学—基础兽医学] S858.292[农业科学—兽医学]