柴油机排出颗粒提取物对细胞间隙通讯的影响  

Effects of Diesel Exhaust Particle on Gap Junction Intercellular Communication

作　　者：宋健[1] 叶舜华[1] 

机构地区：[1]上海医科大学公共卫生学院

出　　处：《卫生研究》1997年第3期145-147,共3页Journal of Hygiene Research

基　　金：国家自然科学基金

摘　　要：利用划痕染料示踪技术（ＳＬＤＴ）将荧光染料引入细胞内，观察细胞间荧光染料的扩散以判断经缝隙连结的细胞间通讯的方法，分析了柴油机排出颗粒物（ＤＥＰ）的提取物对细胞间通讯的影响。结果表明：ＤＥＰ提取物能够明显抑制Ｂａｌｂ／ｃ３Ｔ３细胞间隙通讯，提示ＤＥＰ对细胞间隙通讯的影响可能是其致细胞恶变的原因之一。Scrape loading and dye transfer technique (SLDT) was used to study the effects of diesel exhaust particle (DEP) on gap junction intercellular communication (GJIC). The results demonstrated that the extract of DEP caused inhibition of gap junction intercellular communication in Balb/c 3T 3 cells. It was suggested that inhibition of gap junction intercellular communication in cells may be one of the cause to induce cell transformation.

关 键 词：柴油机 排出颗粒物 细胞间隙通讯 致癌性 

分 类 号：X503.2[环境科学与工程—环境工程] R730.231[医药卫生—肿瘤]