应用PCR-SSCP检测转化细胞中p53基因突变  

Mutation of p53 Gene in the Transformed Fibroblasts Detected by Nonradioisotopic Single Strand Conformation Polymorphism Analysis

作　　者：谭明家[1,2] 王莉娥[1,2] 谢大英[1,2] 李玉瑞[1,2] 方福德 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所 [2]中国医学科学院基础医学研究所

出　　处：《卫生研究》1997年第3期159-161,共3页Journal of Hygiene Research

基　　金：国家自然科学基金;卫生部基金

摘　　要：利用化学致突变物甲基丙烯酸环氧丙酯体外诱导人胚肺成纤维细胞，使之发生转化，分离转化细胞克隆。经ＰＣＲ特异性扩增ｐ５３基因外显子第５和第８，银染单链构象多态性（ＳＳＣＰ）分析，结果表明转化细胞中ｐ５３基因外显子第８发生突变，揭示出ｐ５３基因在化学致突变物诱导细胞转化中起着重要作用。The human embryonic lung fibroblasts transformation was induced by chemical mutant Glycidyl Methacrylate (GMA) in vitro. Transformed clonies were isolated and then exon 5 and exon 8 of p53 gene were specifically amplified by polymerase chain reaction. Amplified fragments were detected by using silver stained single strand conformation polymorphism analysis. The results showed that exon 8 altered in the transformed cells, indicating that p53 gene played an important role in the human embryonic lung fibroblasts transformation induced by GMA .

关 键 词：化学致突变物 细胞转化 P53基因 PCR 

分 类 号：R73－37[医药卫生—肿瘤]