丝裂原素在人单核细胞白细胞介素2受体的表达及杀伤机制中的作用  

Effects of mitogens on the expression of interleukin 2 receptor and the cytotoxicity of human monocytes

作　　者：陈静[1,2] 董海东[1,2] 

机构地区：[1]大连医科大学附属第二医院免疫风湿科 [2]北京医科大学免疫学系T细胞室

出　　处：《中华血液学杂志》1997年第6期291-294,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金

摘　　要：目的：研究丝裂原素在人单核细胞白细胞介素２（ＩＬ－２）受体的表达及杀伤机制中的作用。方法：采用多种丝裂原素处理人单核细胞，观察其对单核细胞ＩＬ－２受体α链表达和杀伤活性的影响。结果：美洲商陆（ＰＷＭ）和脂多糖（ＬＰＳ）在处理２４小时后诱导人单核细胞表达ＩＬ－２受体α链；植物血凝素（ＰＨＡ）只有在处理４８小时后才观察到有诱导α链表达作用；而刀豆素Ａ（ＣｏｎＡ）无明显诱导作用。在以上丝裂原素预处理后，加入ＩＬ－２，在有诱导ＩＬ－２受体α链表达的处理组，可见其单核细胞杀伤肿瘤细胞（Ｕ９３７）的活性明显提高。其中以ＰＷＭ预处理组的单核细胞活性增加最为显著。用流式细胞仪检测到ＰＷＭ可同时与单核细胞和Ｕ９３７靶细胞结合。经ＰＷＭ预处理后，加入ＩＬ－２，明显见到Ｕ９３７细胞发生凋亡的现象（包括核浓缩、凋亡小体的形成和ＤＮＡ的断裂）。结论：通过丝裂原素诱导人单核细胞ＩＬ－２受体的表达，可以促进ＩＬ－２激活的单核细胞的杀伤活性，包括诱导靶细胞的凋亡。Objective: To study the effects of mitogens on the expression of interleukin 2 receptor (IL 2R) and the cytotoxicity of human monocytes.Methods:The effects of mitogens on the regulation of the expression of IL 2R α chain and the cytotoxicity of human monocytes were investigated .Results:Pokeweed mitogen(PWM) and lipopolysaccharide(LPS) induced the expression of IL 2R α chain on human monocytes at 24h and 48h culture,phytohemagglutinin (PHA) did the same only at 48h culture,while concanavalin(ConA) did not.After pretreatment with these mitogens,the addition of IL 2 enhanced cytotoxicity of monocytes only in those with IL 2R expression. The highest IL 2 induced cytotoxicity of monocytes was found in those pretreated with PWM. Flow cytometry showed that PWM bound to the surfaces of both monocytes and U937 target cells.The addition of IL 2 induced apoptosis of PWM pretreated U937 cells.Conclusion: IL 2 enhanced monocyte mediated cytotoxicity and target cell apoptosis, which could be augmented by mitogen induced IL 2R expression on monocytes.

关 键 词：丝裂原素 单核细胞 白细胞介素2 肿瘤 

分 类 号：R730.52[医药卫生—肿瘤]