流式细胞光度术研究大鼠肝癌及癌前病变DNA的变化  

Flow cytometric DNA analysis of hepatic precancerosis and hepatocellular carcinoma in rats

作　　者：郭文栋[1,2] 杨文[1,2] 杜爱萍 左连富[1,2] 

机构地区：[1]山西医科大学第二医院消化科 [2]河北省肿瘤研究所

出　　处：《新消化病学杂志》1997年第5期289-290,共2页

摘　　要：目的用流式细胞光度术（ＦＣＭ）探讨大鼠肝癌和癌前病变ＤＮＡ变化的意义．方法取正常大鼠、肝硬变、非典型增生和肝癌大鼠肝脏组织块，制成单细胞悬液，做ＦＣＭ分析．结果从正常肝组织到肝脏的各级病变，二倍体细胞所占的百分比逐渐增加（７１０±２２，７５０±２８，７６０±４４，８００±３９，Ｐ＜００５），四倍体细胞百分比则下降（１５０±１４，１４０±１０，１２０±２４，９９±１７，Ｐ＜００５），Ｓ期比率（％）升高（８４±１４，１００±１０，１０８±２５，１１３±１８，Ｐ＜００５）．在肝癌大鼠中５０％为异倍体．结论ＦＣＭ分析对肝癌前病变及肝癌的早期诊断有一定的临床意义．AIM To investigate the changes of nuclear DNA content of different hepatic diseases in rats by flow cytometric (FCM) analysis. METHODS Samples were obtained from normal liver, cirrhosis, dysplasia proliferation and hepatocellular carcinoma (HCC) in rats. These samples were made into single cell suspension and analysed with FCM. RESULTS From normal liver to HCC, the diploid ratios were significantly increased (71 0±2 2, 75 0±2 8, 76 0±4 4, 80 0±3 9, P <0 05). The tetraploid ratios were significantly decreased (15 0±1 4, 14 0±1 0, 12 0±2 4, 9 9±1 7, P <0 05), and the S phase fraction ratios increased (8 4±1 4, 10 0±1 0, 10 8±2 5, 11 3±1 8, P <0 05). There were four euploids in the 8 cases of HCC. CONCLUSION FCM DNA analysis is of significance in early diagnosis of HCC.

关 键 词：肝癌 癌前状态 细胞流式术 DNA 

分 类 号：R735.702[医药卫生—肿瘤]