5-烯丙基-7-二氟亚甲基白杨素诱导人肝癌细胞生长抑制和凋亡  

Induction of the Growth Inhibition and Apoptosis in Human Liver Cancer SMMC-7721 Cell Line by 5-ally-7-gen-difluormethylene-chrysin

作　　者：王宇[1] 周秀田[2] 曹建国[2] 周建国[2] 张亮[2] 

机构地区：[1]长治医学院病理科 [2]南华大学肿瘤研究所

出　　处：《长治医学院学报》2007年第3期165-168,共4页Journal of Changzhi Medical College

基　　金：湖南省医药卫生科研基金(B2006-102)

摘　　要：目的:研究5-烯丙基-7-二氟亚甲基白杨素(ADFMChR)对人肝癌(SMMC-7721)细胞生长和凋亡的影响。方法:平皿克隆形成法测定细胞锚定依赖性生长能力;吖啶橙/溴化乙啶(AO/EB)染色荧光显微镜观察凋细胞亡形态,计数细胞凋亡;PI染色流式细胞术(FCM)分析细胞周期和凋亡率。结果:平皿克隆形成法显示:ADFMChR抑制人肝癌(SMMC-7721)细胞生长,且呈浓度依赖性。AO/EB染色荧光显微镜观察发现随着ADFMChR浓度的增加SMMC-7721细胞凋亡率依次增加。PI染色FCM结果表明ADFMChR以浓度依赖方式诱导SMMC-7721细胞凋亡,且使细胞周期阻滞于G_1期。结论:ADFMChR具有诱导人肝癌SMMC-7721细胞凋亡的作用,其机制可能与细胞周期G_1期阻滞相关。Objective：To investigate the effect of 5 - ally - 7 - gen - difluormethylene - chrysin （ADFM- ChR） on the growth and apoptosis of human SMMC- 7721 cells cultured in vitro and its mechanism. Methods： SMMC - 7721 cells were treated with various concentrations of ADFMChR. Cell proliferation of SMMC - 7721 cells was evaluated by clone formation test .The morphology of cell apoptosis of SMMC -7721 cells were detected by AO/EB double fluorescence stain .The apoptosis rate and cell cyle of SMMC- 7721 cells were determined by PI staining flow cytometry. Results： Data of colone formation test indicated that ADFMChR could inhibit the growth of SMMC - 7721 cells in a concentration - dependent manner. PI staining flow cytometry and AO/EB double fluorescence stain showed that cell cyle was arrested at G10 phase and the apoptosis of SMMC - 7721 cells was induced by ADFMChR in a concentration - dependent manner. Conclusions： ADFMChR posseses induction of apoptosis of SMMC- 7721 cells in vitro,and its mechanism might be associated with G1 phase cell cycle arrest.

关 键 词：肝肿瘤 白杨素 细胞周期阻滞 凋亡 

分 类 号：R361[医药卫生—病理学]