两步固态法发酵降解山黧豆毒素的研究  

Reduction Efficiency of the Neurotoxinβ-ODAP in Lathyrus sativus by Two Successive Solid State Fermentation

作　　者：赵兴秀[1] 李志孝[2] 孟延发[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室 [2]兰州大学功能有机分子化学国家重点实验室,甘肃兰州730000

出　　处：《食品与发酵工业》2007年第6期37-40,共4页Food and Fermentation Industries

基　　金：国家自然科学基金(No.30270965)

摘　　要：从保存的菌种中筛选到B_2和B_(13)2株菌,通过2步固态发酵,在山黧豆培养基中先接菌株B_2培养3d (37℃),灭菌后再接菌株B_(13),培养3d(37℃),灭菌后用0.2 mol/L的HClO_4在0～4℃提取1h,然后利用2,4-二硝基氟苯(FDNB)为柱前衍生试剂,通过高效液相色谱(HPLC),检测了对照和处理中α,β-草酰氨基丙氨酸(ODAP)的含量及降解率。结果发现,对照中β-ODAP和α-ODAP平均含量分别为1.146 mg/g和0.653 mg/g,处理中β-ODAP和α-ODAP的平均含量分别为0.203 mg/g和0.0909 mg/g,处理中的β-ODAP和α-ODAP比对照中的β-ODAP和α-ODAP的平均含量降低了82.2%和86%,而且发酵后处理的蛋白含量明显高于对照。Two strains B2 and B13 degraded from the toxin β-ODAP were screened and selected. Two suc cessive solid state fermentations are processed for the two selected strains. After sterilization the seeds were inoculated with B2. The fermentation was carried out at 37℃ for 3 days and was stopped by steam. The second inoculum B13 was added after cooling, and the second fermentation period was processed at 37℃ for 3 days and was stopped by steam. The control and processed seed powders were extracted for 1 hour at 0-4℃ with 0.2mol/L HC104. The contents of α, β-ODAP were measured by precolumn derivatization with 1-fluo- ro-2,4-dinitrobenzene （FDNB）. As a result,the contents of β-ODAP and α-ODAP in control seed powders were 1. 146mg/g and 0. 653 mg/g,respectively. The contents of β-ODAP and α-ODAP processed seed powders were 0. 203mg/g and 0.0909 mg/g. After fermentation, the detoxification efficiency of β-ODAP was 82. 2 % and the content of α-ODAP was decreased by 86 %, and the protein level of the processed was significantly surpassed that of the control.

关 键 词：固态发酵 山黧豆 β-N-草酰基-α β-二氨基丙酸 

分 类 号：TQ914[化学工程]