中性内肽酶及移动相关蛋白-1在恶性黑色素瘤中的表达及意义  

Expression of neutral endopeptidase and motility-related protein-1 in cutaneous malignant melanoma

作　　者：吴艳[1] 张晓冰[2] 刘厚君[1] 郑丽端[3] 李家文[1] 林云[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤科,武汉410022 [2]湖北十堰东风汽车公司茅箭医院皮肤科 [3]华中科技大学同济医学院附属协和医院 病理科,武汉430022

出　　处：《中华病理学杂志》2007年第7期466-469,共4页Chinese Journal of Pathology

摘　　要：目的探讨中性内肽酶(CD10)及移动相关蛋白-1(CD9)在恶性黑色素瘤中的表达及其临床意义。方法采用免疫组织化学 SP 法检测48例原发性皮肤恶性黑色素瘤(CMM)及23例转移性恶性黑色素瘤(转移性 MM)中 CD10、CD9蛋白表达水平,并以23例色素痣作为对照。结果(1)CD10在转移性 MM、CMM 及色素痣中的表达依次减弱,差异均有统计学意义(P<0.01),CD9在转移性 MM 中的表达较 CMM 显著降低(P<0.05);(2)CD9和 CD10在恶性黑色素瘤中的表达水平呈负相关(CMM:r=-0.40,P=0.005;转移性 MM:r=-0.44,P=0.034);(3)CD10和 CD9在CMM 中的表达与病理学类型、淋巴结转移和浸润深度相关;(4)CD10、CD9在 CMM 间质成纤维细胞中的表达呈负相关(r=-0.43,P=0.007),且与肿瘤浸润深度明显相关,与淋巴结转移有一定相关性。结论 (1)CD10及 CD9与恶性黑色素瘤的浸润、转移密切相关,两者在此过程中可能起相互拮抗作用;(2)肿瘤细胞 CD10、CD9的表达对 CMM 的辅助诊断及预后评估具有重要的临床意义;(3)肿瘤间质成纤维细胞中 CD10、CD9的表达在 CMM 的发展过程中亦可能扮演着重要角色。Objective To study the expression of neutral endopeptidase （CD10） and motilityrelated protein-1 （ CD9 ） in malignant melanoma and their clinical significance. Methods Immunohistochemical study for CD10 and CD9 using Streptavidin-biotin complex technique was carried out in 48 cases of primary cutaneous malignant melanoma （ CMM）, 23 cases of metastatic melanoma and 23 cases of benign nevus. Results The positivity rate of CD10 was highest in metastatic melanoma and lowest in benign nevus （P 〈 0. 01 ）. In contrast, the positivity rate of CD9 in metastatic melanoma was lower than that in CMM （ P 〈 0. 05 ）. The expression of CD9 was inversely correlated with that of CD10 in malignant melanoma （ CMM：r = -0. 40,P =0, 005 ;metastatic MM：r = -0, 44,P =0. 034）. The expression of CD10 and CD9 in CMM also correlated with tumor histology, Clark＇s level of invasion and presence of nodal metastasis. A similar relationship was also observed for CD10 and CD9 expression in stromal fibroblasts of CMM（r = -0. 43 ,P = 0. 007）. Conclusions CD10 and CD9 expression correlates with the invasiveness and metastatic potential of malignant melanoma; both factors may demonstrate a counteracting effect. These two markers have potential implications in prognostic assessment of CMM. Stromal fibroblasts may also play an important role in the progression of CMM.

关 键 词：黑色素瘤 免疫组织化学 皮肤 

分 类 号：R739.5[医药卫生—肿瘤]