乳腺癌SLN中CD_(25)^+T及NK细胞的免疫组化研究  

IHC analysis the changes of CD_(25)^+ and NK cell in SLN with breast cancer

作　　者：何建军[1] 任予[1] 霍永平[1] 张云锋[1] 王珂[1] 陈武科[1] 

机构地区：[1]西安交通大学医学院第一附属医院肿瘤外科,陕西西安710061

出　　处：《中国现代医学杂志》2007年第13期1592-1594,1598,共4页China Journal of Modern Medicine

基　　金：陕西省科技攻关项目(2004K13-G5)

摘　　要：目的分析乳腺癌SLN中CD25+T及NK细胞的变化规律。方法从2001年4月～2003年6月所进行的乳腺癌SLNB病例中,选择应用CK19单抗检测到SLN具有微转移的病人12例,并随机选择无转移的9例,HE有转移的10例共82个SLN。将82个SLN分为无转移、微转移及转移组;选择CD25及CD56单抗,采用免疫组化SABC的方法,计数5个高倍视野下阳性染色细胞数和淋巴细胞总数的比值,取其平均值,得出阳性细胞百分比率。结果82个SLN中未转移35个,微转移20个,转移27个;3组淋巴结中CD25+细胞占总淋巴细胞的百分比率在无转移组与微转移组间差异无统计学意义(P=0.260)、而在微转移组与转移组间差异有显著性(P<0.01)。3组SLN中NK细胞占总淋巴细胞的百分比率差异无显著性(F=0.755,P>0.05)。结论在SLN无转移和微转移状态下,CD25+细胞的数量无明显变化,而一旦出现HE转移,则CD25+细胞的数量显著增加。SLN转移与否,NK细胞的数量无明显变化。[Objective] To Analysis dynamically the changes of CD25^＋ and NK Cell in SLN with breast cancer. [Methods] Micrometastasis of SLNs was observed in 12 patients through immunohistochemical methods with CK19 monoantibody. We randomly chosed 9 patients from none-metastasis, and 10 patients from metastasis. Together, 82 SLNs were examined in all patients （31 cases）. Then we tested all samples once more based on routine pathological diagnosis and immunohistochemical results with CK19 monoantibody in order to determine the status of SLNs. Monoantibody of CD25 as well as CD56 and SABC immunohistochemical methods were employed to determine the total number of lymphocytes and dye positive cells in five high scope-fields. The percentage of positive-dye cells was calculated out based on the average number of them. [Results] Among all 82 SLNs, none-metastasis was found in 35 SLNs, micrometastasis in 20 SLNs and metastasis in 27 SLNs, respectively. The ratio of CD25^＋ cells account for total ymphocytes was not different sinificantly between none-metastasis and micrometastasis groups （P =0.260） as well as different between micro-metastasis and metastasis groups （P 〈0.01）. the ratio of CD56^＋ cells didn＇t exhibit significantly difference among three groups （F =0.755, P 〉0.05）, neither did the average. [ Conclusion] The number of CD25^＋ cells would be obviously changed by none-metastasis and micrometastasis status; but be significantly increased by HE-metastasis in SLNs with breast cancer. The number of NK cells would be obviously changed by any metastasis status in SLNs.

关 键 词：乳腺癌 前哨淋巴结 CD25^+T细胞 NK细胞 

分 类 号：R737.9[医药卫生—肿瘤]