羟基喜树碱与吡柔比星联用对膀胱癌细胞株体外生长的影响  

Effect of combined administration of hydroxycamptothecin and pirarubicin on in vitro growth of bladder cancer cell line T24

作　　者：张青川[1] 石家齐[2] 李庆文[1] 

机构地区：[1]蚌埠医学院第一附属医院泌尿外科,安徽蚌埠233004 [2]贵阳医学院附属医院泌尿外科

出　　处：《临床泌尿外科杂志》2007年第7期511-514,共4页Journal of Clinical Urology

摘　　要：目的:探讨羟基喜树碱(HCPT)与吡柔比星(THP)联合用药对膀胱癌细胞的作用。方法:采用MTT法评价HCPT和THP各种联合用药方式对膀胱癌T24细胞生长的抑制情况进行观察,应用流式细胞仪检测各组细胞的凋亡情况。结果:各种给药方式对T24细胞生长均产生抑制作用和致凋亡作用。序贯给药各时点细胞的抑制率和凋亡率除间隔60h外,均高于同时给药(P<0.05)。序惯给药时,于给HCPT后24h时点加药的生长抑制率和凋亡率最高。结论:两药序惯给药可获得协同作用,以先给HCPT后24h时点加THP的抑制作用最强。Objective：To investigate whether there is synergistic action of hydroxycamptothecin （HCPT） and pirarubicin （THP） when combined based on the research on the effect of topoisomerase Ⅰ inhibitor hydroxycampto thecin and topoisomerase Ⅱ inhibitor-pirarubicin on in vitro growth of the bladder cancer cell line T2,1 with different administration patterns. Methods：The proliferation of T 24 cells in all groups was determined with MTT. The apoptosis rate of T 24 cells in all groups was measured with the flow cytometry. Results： All administration pat terns had inhibition effect on the growth of the T24 cells. And all administration patterns could induce the apopto sis of the T24 cells. The inhibition rate and the apoptosis rate of all the sequential groups except the group with the 60 hours interval were higher than those in the simultaneous administration group （ P 〈0.05）. The group of 2,1 hours interval had the highest inhibition rate and highest apoptosis rate comparing with the other groups with different intervals（ P 〈0. 05）. Conclusions： The sequential administration has the higher inhibition effect than the simultaneous administration and the independent administration. The strongest inhibition can be achieved by administering HCPT followed by THP 24 hours later.

关 键 词：膀胱肿瘤 羟基喜树碱 吡柔比星 DNA拓扑异构酶抑制剂 序惯给药 

分 类 号：R737.11[医药卫生—肿瘤]