我国分离的盖塔病毒衣壳蛋白基因和3′非翻译区分子特征研究  

Molecular Analysis on the Capsid Gene and 3′ Untranslation Region of Three Getah Viruses Isolated in China

作　　者：翟友刚[1] 王焕琴[1] 付士红[1] 梁国栋[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京100052

出　　处：《病毒学报》2007年第4期270-275,共6页Chinese Journal of Virology

摘　　要：对我国海南省和河北省分离到的3株盖塔病毒（GETV）（M1、HB0215-3和HB0234）进行衣壳蛋白基因和3′UTR区序列测定,并分析比较该病毒的分子生物学遗传特征。首先应用逆转录聚合酶链反应扩增出病毒衣壳蛋白基因和3′UTR片段,纯化后连接到载体中进行测序,然后用Clastal X和DNASTAR软件对测定的核苷酸和推测的氨基酸序列进行比较分析,用MEGA软件绘制系统发生树。3株病毒衣壳蛋白基因分别由801、804和804个核苷酸组成,分别编码267、268和268个氨基酸,3株病毒之间核苷酸和氨基酸序列同源性为97.6%-100%和97.8%-100%,与其他GETV分离株核苷酸同源性在95.4%-99.6%之间。3株病毒3′UTR分别由411、401和401个核苷酸组成,发现中国株存在10个（45-54位）核苷酸缺失和2个（64位、148位）特有的核苷酸位点。进化分析表明盖塔病毒之间的进化关系与分离年代相关,中国境内流行的盖塔病毒是相对独立的一个类群。To compare the molecular characteristics of the Capsid gene and 3＇ untranslation region （3＇UTR） of Getah viruses （GETV） isolated in Hainan Province and Hebei Province of China,the viral RNAs were extracted from M1（Hainan）, HB0215-3 and HB0234（Hebei） virus stocks. Capsid gene segments and 3＇ UTR segments from three strains of Chinese GETV were obtained by RT-PCR and then sequenced. The obtained nucleotide sequences were analyzed using the Clustal X（1.8）, DNASTAR, MAGA3.1 programs. The full-length Capsid gene of the 3 strains of Chinese GETV were comprised of 801, 804 and 804 nucleotides each, encoding the protein of 267,268 and 268 amino acids each. Sequencing of Capsid gene fragments showed that two strains of Hebei isolates were identical and had homology of 97.6 % at nucleotide level and 97.8% at amino acids level with M1. Their homologies when compared with strains isolated from other countries were also high at nuc.leotide levels （95.4%-99.6%）. The 3＇UTR from the three strains were comprised of 411, 401 and 401 nucleotides each, and had found specific deletion of 10 nt at position 44-54 and two specific nucleotide sites that was T at position 64 and C at position 148. GETV isolated in China presented relation of the year of virus isolation with the phylogenesis distance when compared with the other GETV strains and comprised a genetically highly conserved group.

关 键 词：盖塔病毒 衣壳蛋白基因 3′非翻译区 核苷酸序列 系统发生树 

分 类 号：R373.3[医药卫生—病原生物学]