在我国发现普马拉病毒新亚型  

Identification of a New Puumala Hantavirus Subtype in Rodents from China

作　　者：唐丽华[1] 张全福[1] 修梅红[1] 扈光伟[2] 沈博[2] 杨显达[2] 梁米芳[1] 李德新[1] 

机构地区：[1]传染病预防控制国家重点实验室中国疾病预防控制中心病毒病预防控制所,北京100052 [2]吉林省疾病预防控制中心,长春130062

出　　处：《病毒学报》2007年第4期320-325,共6页Chinese Journal of Virology

摘　　要：对我国东北地区捕获的157份棕背鼠平的肺组织进行普马拉病毒检测。其中免疫荧光检测出阳性标本1份,PCR检测出核苷酸阳性标本7份。对PCR产物进行测序后发现,其核苷酸的序列与报道的普马拉病毒核苷酸序列存在着差异。系统进化分析表明,我国发现的这株普马拉病毒,在进化树上形成了一个新的分支,为一新亚型。并且与在韩国和日本发现的普马拉病毒亲缘关系最为接近。In order to detect the nucleic acid of Puumala hantavirus, RNA was extracted from lungs of bank voles captured in Northeast China , and partial S and M genome segments of Puumala virus were amplified by RT-PCR and sequenced. Phylogenetic analysis suggested that Chinese Puumala virus had diverged from the common node of PUUV, with accumulating nucleotide substitutions and formed a distinct lineage from other Puumala viruses. Newly found Puumala virus was most closely related to the Kamiiso-8Cr-95 and Tobetsu-60Cr-93 strains which came from Japan and the muju strains which came from South Korea. By analysis of S and M genome segments of Puumala virus, we deduced a new Puumala virus subtype did exist in Northeast China.

关 键 词：普马拉病毒 S片段 M片段 核苷酸序列分析 

分 类 号：R373[医药卫生—病原生物学]