人类真核翻译延长因子1A2在胰腺癌中抑制凋亡致癌机制研究  

Human eukaryotic translation elongation factor 1 alpha 2 suppresses apoptosis in pancreatic cancer

作　　者：诸琦[1] 张愫[1] 曹海霞[1] 蔡驹[1] 高耀博[1] 章永平[1] 徐凯[1] 齐充[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院消化科,200025

出　　处：《中华消化杂志》2007年第6期385-388,共4页Chinese Journal of Digestion

基　　金：上海市科委自然科学基金(04ZR14071)

摘　　要：目的探讨人类真核翻译延长因子1A2(Homo sapiens eukaryotic translation elongation factor 1 alpha 2,EEF1A2)可能的致癌机制。方法培养人胰腺导管细胞腺癌细胞株BxPC-3;采用EEF1A2 siRNA干扰EEF1A2高表达的胰腺癌细胞株BxPC-3,另设空白对照组和阴性对照组。采用Western印迹检测各组EEF1A2蛋白水平变化;四甲基偶氮唑盐(MTT)法检测各组细胞增殖抑制率;分别应用流式细胞仪、透射电镜以及末端脱氧核苷酰转移酶原位标记(TUNEL)法检测细胞凋亡。结果靶向EEF1A2的序列特异性的siRNA可以高效地抑制BxPC-3细胞EEF1A2基因表达。EEF1A2 siRNA干扰细胞24、48 h后,与阴性对照组相比,实验组细胞的细胞增殖被显著抑制(23.51%比37.67%和11.53%比48.89%,P<0.05),凋亡明显增加[(2.820±0.240)%比(8.505±2.454)%和(2.850±1.117)%比(4.075±1.068)%。P<0.05].TUNEL标记分析和透射电镜均发现典型凋亡特征。结论EEF1A2可能是胰腺癌的一个癌基因。EEF1A2可能通过抑制细胞凋亡途径促进胰腺癌细胞生长。Objective To explore the potential mechanisms of carcinogenesis for human eukaryotic translation elongation factor 1 alpha 2 （EEF1A2）. Methods Specific inhibition of EEF1A2 with siRNA was achieved in human pancreatic cancer cell line, BxPC-3, which usually expresses high level of EEF1A2. The changes of EEF1A2 expression were determined by Western blot. The effect of siRNA in suppressing the proliferation of BxPC-3 cells was determined by MTT assay, and its role in inducing BxPC-3 cell apoptosis evaluated by flow cytometry, TUNEL and transmission electron microscope. Results The sequence-specific siRNA effectively suppressed the expression of both EEF1A2 mRNA and protein. Specific inhibition of EEF1A2 with siRNA in pancreatic cancer cell line BxPC-3 could suppress proliferation and induce apoptosis. Conclusion The oncogenicity of EEF1A2 may be related to its role in suppressing the apoptosis and promoting the growth of pancreatic cancer cells.

关 键 词：转录延伸因子 胰腺癌 RNA干扰 细胞凋亡 

分 类 号：R735.9[医药卫生—肿瘤]