体外药物敏感试验ATP-TCA在肝癌化疗中的应用  被引量:1

Application of ATP base bioluminescence tumor chemosensitivity assay in the chemotherapy of hepatocellular carcinoma

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作  者:李立志[1] 郭雅[2] 黎乐群[2] 李佳荃[3] 

机构地区:[1]广西民族医院肝胆外科 [2]广西医科大学第一附属医院肝胆外科 [3]广西医科大学实验中心,广西南宁530000

出  处:《华夏医学》2007年第3期430-432,共3页Acta Medicinae Sinica

摘  要:目的:探讨体外三磷酸腺苷生物荧光法化疗药物敏感试验(ATP-TCA)在肝癌化疗中的应用价值。方法:用ATP-TCA法检测6种化疗药物对37例肝细胞癌标本的敏感率和耐药率。结果:37例肝癌标本中,ATP-TAC的可评估率为89.19%,6种化疗药物的敏感率分别为:更生霉素(ACTD)84.85%;长春瑞滨(NVB)78.79%;丝裂霉素C(MMC)75.76%;羟基喜树碱(HCT)42.42%;长春新碱(VCR)39.39%;吡柔比星(THP)36.36%。各试验药物的敏感率与肿瘤的大小、血清甲胎蛋白(AFP)无显著相关性(P>0.05),ACTD在P53蛋白阴性时敏感性较好,而ACTD、HCT、THP对、级肝癌的敏感率高于、级。结论:ATP-TCA法在检测肝癌化疗药物敏感性时稳定可靠,可作为肝癌化疗药物筛选的方法之一。Objective: To investigate the value and application of ATP base bioluminescence tumor chemosensitivity assay (ATP-TCA) in the chemotherapy of hepatocellular carcinoma. Methods : Six anticancer chemotherapeutic agents were tested by using ATP-TCA with regard to cytotoxicity on treating 37 specimens of hepatocellular carcinoma resected from surgery. Results : The outcomes of 33 in thirty-seven specimens were evaluable with an overall evaluation rate of 89.19%. The sensitivity rate of Dactinomycin (ACTD), Vinorelbine (NVB), Mitomycin C (MMC), Hydroxycamptothecin (HCT), Vincristine(VCR), Thio-Tepa (THP) were 84. 85%, 78. 79%, 75. 76%,42. 42%, 39. 39%, 36.36%, respectively. The result of chemosensitivity assay showed no correlation with tumor's size and serum α-fetoprotein level. The sensitivity rate of ACTD was higher when protein p53 were negative comparing to those positive cases while which of ACTD, HCT,THP were correlatied to pathologic degree. Conclusion: ATP-TCA can be used in testing chemotherapeutic drugs against liver cancer.

关 键 词:肝细胞癌 化疗药物敏感试验 三磷酸腺苷生物荧光试验 

分 类 号:R735.7[医药卫生—肿瘤]

 

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