应用PCR检测鸡传染性喉气管炎病毒  

Detection of Infectious Laryngotracheitis Virus with Polymerase Chain Reaction

作　　者：陈红英[1] 李新生[1] 张红英[1] 方忠意[2] 王东方[1] 崔保安[1] 

机构地区：[1]河南农业大学牧医工程学院 [2]河南省兽药监察所,郑州450002

出　　处：《中国畜牧兽医》2007年第7期83-85,共3页China Animal Husbandry & Veterinary Medicine

基　　金：国家"十五"食品安全重大攻关专项(2001BA804A30-11)

摘　　要：根据传染性喉气管炎病毒（ILTV）TK基因序列,设计、合成1对引物,应用PCR技术对ILTV以色列疫苗株、河南分离株（ILTV-CG和ILTV-XY）进行PCR扩增,均能扩增出预期大小的目的片段,测序分析和酶切分析证实了PCR产物的特异性,而对其它禽病原体的扩增均为阴性。PCR检测ILTV DNA的最小检测量为21 pg。应用PCR检测人工接种后不同天数采集的鸡的结膜拭子,接种后第2-5 d均能检测到ILTV。该方法可用于鸡传染性喉气管炎病的诊断和临诊样品检测。One pair of primers was designed and synthesized according to the chicken infectious laryngotracheitis virus （ILTV） TK gene nucleotide sequence in the Genbank. When PCR was performed with 2 virulent ILTV,1 vaccine ILTV and other pathogenic microorganisms, the amplified fragment of ILTV was about 1183 bp which could be confirmed by sequencing analy- sis and restriction endonuclease analysis, others were negative. 21 pg ILTV DNA could be detected by PCR. All of the tracheal sponge samples from experimentally infected chickens was amplified a specific 1183 bp fragment of TK gene. The results suggested that the method can be used for detection and diagnosis of ILTV in clinic samples.

关 键 词：鸡传染性喉气管炎病毒 检测 PCR 

分 类 号：S852.659.1[农业科学—基础兽医学]