地中海实蝇幼虫分子鉴定  

Molecular identification for larvae of Ceratitis capitata

作　　者：叶军[1] 周国梁[1] 易建平[1] 吴家教[2] 阮长浩[3] 梁帆[2] 郑建中[1] 王文兵[3] 

机构地区：[1]上海出入境检验检疫局,上海200135 [2]广东出入境检验检疫局,广州510623 [3]江苏大学生命科学院,镇江212013

出　　处：《昆虫知识》2007年第4期562-566,共5页Entomological Knowledge

基　　金：上海市重大科技攻关项目(03DZ19315和04DZ05002);国家质检总局科技专项(2004IK046)

摘　　要：通过对从秘鲁进口的葡萄中截获实蝇类幼虫进行ITS区和线粒体COⅠ,COⅡ,COⅢ、ND5基因序列的扩增和测序,并与GenBank中对应的序列进行比对,结果表明,截获样品ITS区序列和地中海实蝇Ceratitis capitata(Wiedemann)同源性为95·16%(其中ITS1为99·52%,ITS2为86·2%),线粒体COⅠ,COⅡ,COⅢ,ND5基因序列和地中海实蝇C.capitata同源性为100%,99·9%,99·5%,99·8%;基于COⅠ序列构建的系统发育树中,幼虫样品和地中海实蝇最为接近。根据序列分析和系统发育关系分析的结果,将截获的实蝇类幼虫鉴定为地中海实蝇C.capitata。The internal transcribed spacer （ITS） region and mitochondrial gene, including the mitochondrial cytochrome oxidase subunit I （CO I ） gene, CO Ⅱ , CO Ⅲ and NADH dehydrogenase （ubiquinone） chain 5（ND5）, of larvae（PU-1） intercepted in tablegrape imported from Peru in Mar., 2006, were amplified and sequenced with primers designed from Ceratitis capitata （Wiedemann） （AF189691,AJ242872） and Bactrocera oleae （AY210702, AY210703）. The ITS region （DQ490237）, CO I （ DQ456861 ）, CO Ⅱ （DQ456862）, CO HI （DQ456863） and ND5 （DQ456864） gene sequence were obtained and analyzed with the homologous sequence of related species in GenBank. The result showed that the similarity of ITS is 95.16% （1633/1716） between PU-1 and C. capituta AF189691, The similarity of CO I , CO Ⅱ, CO HI and ND5 gene sequence are 100% （1475/1475）, 99.9% （686/687）, 99.5% （785/789）, 99.8（563/564） between PU-1 and C. capitata CCA242872, respectively. The molecular phylogenetic relationship of 22 species of Tephritidae based on CO I gene sequences showed that PU-1, C. capitata CCA242872, DQ011888, DQ006887, AY788415 and C. caeteate AY788414 are in the same elade. The larvae（PU-1 ） was identified as C. capitata with the analysis of sequence and phylogenetie relationship.

关 键 词：地中海实蝇 幼虫 序列分析 系统发育关系 鉴定 

分 类 号：S433[农业科学—农业昆虫与害虫防治]