头颈部非霍奇金淋巴瘤患者T细胞亚群和自然杀伤细胞检测及其临床意义  

Measurement of T Lymphocyte Subgroup and Natural Killer Cell in Patients with Non-Hodgkin Lymphoma in the Head and Neck and Its Clinical Significance

作　　者：徐刚[1] 张剑波[2] 王槐富[1] 杜泽秀[1] 

机构地区：[1]四川省医学科学院四川省人民医院耳鼻喉科,成都610072 [2]四川省人民医院中心实验室,成都610072

出　　处：《中国眼耳鼻喉科杂志》2007年第4期217-219,共3页Chinese Journal of Ophthalmology and Otorhinolaryngology

摘　　要：目的观察头颈部非霍奇金淋巴瘤患者 T 细胞亚群和自然杀伤细胞(natural killer cell,NK cell,NK 细胞)的变化,并探讨其临床意义。方法流式细胞仪和免疫速率比浊法测定不同阶段的65例头颈部非霍奇金淋巴瘤患者外周血 T 细胞亚群和 NK 细胞、CD19^+B 细胞和免疫球蛋白(immunoglobulin,Ig)以及补体C3,C4的含量,并与25例健康人的相应指标做比较。结果 (1)初发和复发阶段除 CD8^+增高外,CD3^+,CD4^+,CD4^+/CD8^+比值均明显低于健康对照组(P<0.05);缓解期患者上述免疫学指标恢复正常(其中初发 CD8^+,复发 CD4^+,CD4^+/CD8^+P<0.05,其余 P<0.01);(2)所有患者 CD19^+B 细胞,均显著减少(P<0.01);(3)初发期患者NK细胞低于健康对照组(P<0.05),其余各阶段与健康对照组比较,差异均无统计学意义;(4)初发和复发阶段,与健康对照组相比,IgA,IgG 增高(P<0.05),C3降低(P<0.05),而 IgM 和 C4在各阶段,变化均无统计学意义(P>0.05);(5)初发和复发阶段 CD3^+,CD4^+,CD8^+和 CD4^+/CD8^+比值,初发阶段 NK 细胞均与 IgA,IgG,C3有相关性,与 IgM,C4无相关性。结论头颈部非霍奇金淋巴瘤患者细胞免疫功能受到抑制,T细胞亚群及 NK 细胞的检测对该病的诊断、治疗及预后判断有一定的临床价值。(中国眼耳鼻喉科杂志,2007,7:217-219)Purpose To investigate changes in T lymphocyte subgroup and natural killer cell（NK cell） in patients with non-Hodgkin lymphoma （NHL） in the head and neck and its clinical significance. Methods The T lymphocyte subgroups, NK ceils, CD19^＋ B lymphocytes, immunoglobulin （Ig）, complement of C3 ,C4 content of 65 patients with NHL and 25 normal subjects were determined with flow cytometer （FCM） and immunoturbidimetry respectively. Results （1） The number of CD3^＋ ,CD4^＋ and CD4^＋/CD8^＋ratio reduced markedly （P〈0.05 或 P 〈0.01） at the initial and recurrent stage, with the exception of CD8^＋ increasing, and returned to normal level at the remissive stage. （2） The CD19^＋ B lymphocytes in all patients were much fewer than those in the controls（P 〈 0.01 ）. （3）There was no significant difference in NK cells at the remissive and recurrent stage between the patients and the controls except a reduction at the initial stage. （4）The content of IgA, IgG increased while C3 decreased at the initial or recurrent stage compared with the controls（P 〈0.05）. There were no significant changes in content of IgM and C4 at all stages. （5）The number of CD3^＋ , CD4^＋ , CD8^＋ and CD4^＋/CD8^＋ ratio at the initial or recurrent stage and NK cells at the initial stage correlated with content of IgA, IgG and C3 （P〈0.01） but not with content of IgM and CA. Conclusion The cellular immune function in patients with NHL in the head and neck is suppressed significantly. Measurement of lymphocyte subgroups and NK cells is of value in diagnosis, treatment and prognosis of NHL in the head and neck.

关 键 词：非霍奇金淋巴瘤 头颈部 T细胞亚群 NK细胞 流式细胞仪 免疫球蛋白 

分 类 号：R739.91[医药卫生—肿瘤]