实验性变态反应性脑脊髓炎大鼠大脑Olig基因表达的变化规律  

Olig expression in rats of experimental allergic encephalomyelitis

作　　者：余洪俊[1] 费军[2] 刘宏亮[1] 汪琴[1] 姚忠祥[3] 

机构地区：[1]第三军医大学西南医院康复科,重庆400038 [2]第三军医大学大坪医院野战外科研究所全军战创伤中心,创伤,烧伤与复合伤国家重点实验室,重庆400042 [3]第三军医大学基础医学部组织学与胚胎学教研室,重庆市神经科学研究所,重庆400038

出　　处：《第三军医大学学报》2007年第15期1482-1484,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(30572364)~~

摘　　要：目的研究实验性变态反应性脑脊髓炎(experimental allergic encephalomyelitis,EAE)大鼠大脑Olig1,Olig2和Nkx2.2基因的表达规律。方法采用注射豚鼠脊髓匀浆的方法制作Wistar大鼠EAE模型。实时RT-PCR检测基因表达的相对值。结果正常大鼠大脑Olig1,Olig2和Nkx2.2都低水平表达。EAE大鼠症状发作后第6天,其表达水平达最高峰,与正常组比较,相差显著(P<0.05)。三者的变化规律一致。结论Olig基因表达量的增高程度可能是EAE大鼠髓鞘修复不完全的因素之一。Objective To study the experessions of Olig1, Olig2 and Nkx2.2 in th brain tissues of rats of experimental allergic encephalomyelitis （EAE）. Methods The Wistar rats were immunized with guinea pig＇ s homogenated spinal cord mixed with CFA. The expressions of the three genes were detected by real-time PCR. Results In normal rats, Olig1, Olig2 and Nkx2.2 expressed in low level. On day 6 after onset of the symptoms of EAE, the experessions of Olig1, Olig2 and Nkx2.2 reached the peak value, with significant difference with those of normal rats （ P 〈 0.05）. Conclusion The increasing extent of Olig1, Olig2 and Nkx2.2 is related to incomplete remyelination in EAE.

关 键 词：实验性态反应性脑脊髓炎 Olig1 OLIG2 Nkx2.2 

分 类 号：R-332[医药卫生] R394.3